壶腹癌中P16蛋白及PCNA表达的临床意义

目的：探讨壶腹癌中抑癌基因Ｐ１６蛋白与增殖细胞核抗原（ＰＣＮＡ）表达的相关性及其与肿瘤临床病理特征的关系。方法：采用免疫组织化学ＡＢＣ法检测４５例壶腹癌及１１例癌旁正常胰腺组织中Ｐ１６蛋白及ＰＣＮＡ的表达，并与临床病理学进行对比分析。结果：Ｐ１６蛋白在壶腹癌和癌旁正常胰腺组织中的阳性表达率有显著性差异（Ｐ＜０．０５）；Ｐ１６蛋白表达阳性与是否侵犯胰头及肿瘤大小无关；Ｐ１６蛋白与ＰＣＮＡ的表达呈明显

Expression of P16 protein and PCNA in ampullary carcinoma and clinical implication

Objective:To study the expression of P16 protein and PCNA in ampullary carcinoma and its relation with its pathological features. Method: P16 and PCNA expression was immunohistochemically detected in 45 cases of ampullary carcinoma cases and 11 cases of normal pancreatic tissues adjacent to neoplastic lesions. The data were analyzed in connection with clinical findings. Results: The positive rate of P16 protein was higher in nonneoplastic tissues than in tumor tissues ( P <0.05),the positive expression of P16 protein was correlated with low tumor differentiation degree,invasion to pancreas,lymph node metastasis and the TNM higher stages. The positive expression rate of PCNA in tumor tissues was significantly higher in patients with lymph node metastasis( P <0.05) and was not correlated with invasion and tumor size. Conclusion:The abnormal expression of P16 and PCNA are closely correlated with the occurrence ,development and malignancy of ampullary carcinoma.P16 and PCNA may serve as markers in the evaluation of the malignancy of ampullary carcinoma.