重组人骨形成蛋白2-珊瑚复合人工骨修复骨质缺损的实验研究

目的弥补珊瑚无骨诱导活性、成骨能力弱等缺陷。方法将珊瑚与重组人骨形成蛋白２（ｒｅｃｏｍｂｉｎａｎｔｈｕｍａｎｂｏｎｅｍｏｒｐｈｏｇｅｎｅｔｉｃｐｒｏｔｅｉｎ－２，ｒｈＢＭＰ－２）复合，制备出ｒｈＢＭＰ－２－珊瑚复合人工骨。将其植入兔颅骨直径１５ｍｍ的圆形缺损，以单纯珊瑚植入作对照。术后２、６、１２周取材，通过组织学观察和计算机图像分析，评价其骨修复能力。结果ｒｈＢＭＰ－２－珊瑚复合人工骨以传导成骨和诱导成骨双重机制完成骨修复过程，骨修复能力和骨修复效果明显优于单纯的珊瑚。结论此复合人工骨是一种较理想的骨移植替代材料。

An experimental study on the bone repairing ability of recombinant human bone morphogenetic protein 2 coral composited artificial bone

Objective To improve the bone repairing ability of coral. Methods Recombinant human bone morphogenetic protein 2 (rhBMP 2) coral composited artificial bone was used. The composite was implanted into rabbit calvarial critical size defects, and coral alone was implanted as control. The rabbits were sacrificed at 2,6, 12 weeks respectively after operation. The bone repairing ability was assessed by histology and image analysis system. Results The composite repairing the bone defect both in osteoconductive and osteoinductive manners was seen, repairing ability and effect were better than those of coral. Conclusion The composite is ideal as a bone graft substitute.