Bio-oss结合Bio-gide修复牙种植体周围骨缺损的组织学研究

目的：通过制作带有种植体的硬组织磨片，评价无机牛骨(deproteinized natural bovine mineral，Bio-oss)结合可吸收性胶原膜(bioresorbable collagen mambrane，Bio-gide)在牙种植体周围骨缺损中的引导骨再生作用及效果。方法：在免股骨植入羟基磷灰石涂层BLB种植体，并在其外侧壁制造标准骨缺损，A组在骨缺损处植入Bio-oss颗粒并在其表面覆盖Bio-gidt，膜，B组作为空白对照，分别于术后1、2、4、6个月取样品，通过带种植体的硬组织切片进行骨组织形态学分析。结果：术后1个月Bio-oss颗粒表面有新骨形成，随时间延长Bio-oss发生降解吸收，新生骨量增加，并与种植体表面形成骨性结合。结论：可吸收性胶原膜Bio-gide结合Bio-oss应用于牙种植体周围骨缺损中，可以引导骨组织再生，重建缺损的骨组织，新生骨与种植体形成骨性结合。

Histological Studies on the Regeneration of Bone Defect in Dental Implant with Bio-oss and Bio-gide

Objective: The aim was to evaluate effectiveness of deproteinized natural bovine mineral(Bio-oss)with bioresorbable collagen mambrane(Bio-gide), for guided bone regeneration in bone defect around implants. Methods: Fixtures coated by HA were implanted. Standard opening bone defect was made on one side of every fixture. Rabbits in group A were implanted with Bio-oss and covered with Bio-gide on surface. The rabbits in group B were negative contrast . In the 1,2,4,and 6 postoperative months, The animals were killed and sampled, then hard-tissue sections with implants were produced for histological analysis. Results: New bone formation occurred along surface of Bio-oss after one month, newly formed bone were increased with time and contacted to the surface of the implants. Conclusion: Bio-oss combining with Bio-gide can guide the bone regeneration and repair bone defect around implant. New bone was contacted to the surface of implants, and osseointegration was formed.