多发性硬化患者外周血CD8+记忆性T细胞亚群效应细胞因子的表达及其与病情严重程度的相关性

目的 探讨多发性硬化患者外周血CD8+记忆性T细胞亚群效应细胞因子穿孔素和颗粒酶-B的表达及其与病情严重程度的相关性.方法 采用扩展的残疾状况量表(EDSS)对19例多发性硬化患者的病情进行评估,采用流式细胞仪和直接免疫荧光法检测其CD8+记忆性T细胞亚群的数量,同时选择20例健康体检者作为对照.结果 多发性硬化患者组CD8+效应记忆性T细胞和终末效应记忆性T细胞的数量均明显少于健康对照组,差异有显著性(P＜0.05).CD8+效应记忆性T细胞的数量与病情的严重程度呈负相关.结论 CD8+效应记忆性T细胞和终末效应记忆性T细胞的数量可作为一项评估多发性硬化病情严重程度的指标.

Expressions of cytotoxity-related cytokines in CD8~+ memory T cell subsets in patients with multiple sclerosis and correlation between the cytokine levels and disease severity

【Objective】 To investigate the expressions of perforin and granzyme-B in CD8+ memory T cell subsets of peripheral blood in patients with multiple sclerosis and correlation between the cytokine levels and disease severity.【Methods】 The disease severity of 19 patients with untreated multiple sclerosis was reviewed using the extended disability status scale(EDSS).Flow cytometry and direct immunofluorescence assay were employed to detect the number of CD8+ memory T cell subsets which expressed perforin and granzyme-B in peripheral blood of patients with multiple sclerosis and healthy controls.【Results】 The number of effector memory CD8+ T cells and terminal effector memory CD8+ T cells in multiple sclerosis patients group were lower than that in healthy control group,with statistically significant difference(P <0.05).The number of effector memory CD8+ T cells was negatively correlated to disease severity of multiple sclerosis.【Conclusion】 Effector memory CD8+ T cells and terminal effector memory CD8+ T cells can be used as an assessment indicator of the severity of multiple sclerosis.