HPLC同时测定参附汤中新乌头碱、乌头碱、次乌头碱含量的研究

[目的]建立参附汤中新乌头碱、乌头碱、次乌头碱的含量测定方法。[方法]采用Hypersil BDS C18(4.6mm×250mm,5μm)色谱柱,柱温25℃,流动相为甲醇、0.1%三乙胺,梯度洗脱,流速:1.0mL.min-1,检测波长237nm。[结果]新乌头碱在0.005~0.5mg.mL-1范围内线性关系良好,r=0.9999,平均回收率98.49%,相对标准偏差(RSD)为2.31%;乌头碱在0.005~0.5mg.mL-1范围内线性关系良好,r=0.9999,平均回收率99.88%,RSD为1.57%;次乌头碱在0.005~0.5mg.mL-1范围内线性关系良好,r=0.9999,平均回收率99.67%,RSD为1.75%。[结论]该方法学考察符合定量要求,结果准确,可用于同时测定参附汤中新乌头碱、乌头碱、次乌头碱的含量,为参附汤的质量控制提供依据。

Simultaneous Determination of Mesaconitine, Aconitine, Hypaconitine in ShenFu Decoction by HPLC

[ Objective ] To establish a method for the determination of mesaconitine, aconitine, hypaconitine in ShenFu decoction. [ Method] A Hypersil BDS C18 column（4.6mm×250mm, 5μm） was used at 25℃, and the mobile phase was consisted of methanol and 0.1% triethylamine for gradient elution with the flow rate 1.0 mL .min ^-1. The detection wavelength was 237nm, [Result] The linearity ranges for mesaconitine, aconitine, hypaconifine were 0.0{）5-0.5 mg.mL-1 （r=0.9999）, 0.005～0.5 mg.mL-1 （r=0.9999） and 0.005～0.5 mg-mL ^-1 （r=0.9999）, respectively. The average recoveries were 98.49% with relative standard deviation（RSD） 2.31%, 99.88% with RSD 1.57%, 99.67% with RSD 1.75%. [Conclusion] The quantitative method was feasible and beneficial for the quahty control of ShenFu decoction.