三七总皂苷联合阿糖胞苷对K562细胞的影响

目的]考察三七总皂苷联合应用阿糖胞苷对白血病细胞K562细胞活力、增殖抑制、诱导凋亡的协同作用.方法]处于对救生长期的白血病K562细胞,给予三七总皂苷、阿糖胞苷以及两药的联合,采用MTT试验、集落形成实验、Annexin V-PI双染流式细胞检测等实验手段进行分析检测.结果]单用三七总皂苷对K562白血病细胞有一定的增殖抑制作用,对抑制集落形成和诱导凋亡作用不明显；相同剂量的三七总皂苷联用阿糖胞苷后,对K562细胞的增殖抑制、集落形成和凋亡有明显的增强.结论]三七总皂苷联用阿糖胞苷对白血病细胞K562有明显的抗肿瘤作用,提示三七总皂苷可提高白血病细胞对化疗药物的敏感性,为临床用三七总皂苷抗白血病的辅助治疗提供依据.

The Effect of Combined Administration of Panax Notoginseng Saponins and Cytarabine on K562 Cells

Objective] In order to investigate Panax notoginseng saponins combined with cytarabine in leukemia K562 cell viability,proliferation inhibition and synergistic induction of apoptosis.Methods] Leukemia K562 cells in the logarithmic growth phase,given Panax notoginseng saponins,cytarabine,and two-drug combination,using the MTT assay,colony formation assay,Annexin V-PI double staining flow cytometry experimentsanalysis and detection.Results] Single Panax notoginseng saponins on K562 cells proliferation inhibition,inhibition of colony formation and induction of apoptosis is not obvious;the same dose of Panax notoginseng saponins combined with cytarabine on K562 cellsinhibition of proliferation,colony formation and apoptosis are greatly enhanced.Conclusion] PNS associated with cytarabine leukemia K562 cells has significant anti-tumor effect,suggesting that Panax notoginseng saponins can increase the sensitivity of leukemia cells to chemotherapeutic drugs,and provide the basis for the clinical use of adjuvant therapy of anti-leukemia.