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| 食管癌组织中线粒体DNA突变检测 | |
| 引用本文： | 宋谦,张世杰,王明臣,高丽,王志举,赵国强,董子明. 食管癌组织中线粒体DNA突变检测[J]. 郑州大学学报(医学版), 2005, 40(4): 601-603 |
| 作者姓名： | 宋谦 张世杰 王明臣 高丽 王志举 赵国强 董子明 |
| 作者单位： | 1. 郑州大学基础医学院病理生理学教研室,郑州,450052 2. 郑州大学基础医学院微生物学与免疫学教研室,郑州,450052 3. 郑州大学基础医学院生物化学教研室,郑州,450052 4. 河南省人民医院儿科,郑州,450003 5. 郑州大学基础医学院生理学教研室,郑州,450052 |
| 基金项目： | 国家自然科学基金资助项目39870287 |
| 摘 要： | 目的：检测食管癌组织中线粒体DNA(mtDNA)的突变。方法：提取8例食管癌组织的mtDNA；PCR扩增mtDNA的控制区，纯化PCR产物、与T载体连接，转化人肠杆菌，筛选出阳性克隆，用引物T7／SP6做PCR鉴定后测序，与GenRank序列对比。结果：8例标奉mtDNA的控制区共有36个位点发生突变，其中转换32个，颠换4个；同义突变13个，错义突变23个。结论：mtDNA突变很可能在食管癌的发生中发挥一定作用。 |
| 关 键 词： | 食管肿瘤 线粒体DNA D—loop区 突变 |
| 修稿时间： | 2005-03-10 |
Detection of mitochondrial DNA mutations in esophageal cancer tissue |
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| SONG Qian,Zhang Shijie,WANG Mingchen,GAO Li,WANG Zhiju,ZHAO Guoqiang,DONG Ziming. Detection of mitochondrial DNA mutations in esophageal cancer tissue[J]. Journal of Zhengzhou University: Med Sci, 2005, 40(4): 601-603 | |
| Authors: | SONG Qian Zhang Shijie WANG Mingchen GAO Li WANG Zhiju ZHAO Guoqiang DONG Ziming |
| Abstract: | Aim: To detect the mitochondrial DNA(mtDNA) mutations in esophageal cancer tissue. Methods: mtDNA was extracted from tissue of 8 patients with esophageal cancer. D-loop region was amplified using PCR. The PCR products were purified, and linked with T-vector and transformed into JM109. The positive clone was screened and identified by primer T7/SP6 and then sequenced. The mtDNA mutations in the tumor tissue were compared with the GenBank sequence. Results: Mutations were found in all specimens.Totally 36 sites of mutation were found,including 32 sites of coversion,and 4 sites of transversion;13 sites of synonymous mutation and 23 sites of missense mutation. Conclusion: mtDNA mutation probably plays a role in the development of esophageal cancer. |
| Keywords: | esophageal neoplasm mitochondrial DNA D GAAB2 loop region mutation |
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