ShockProtein40在肝内胆管癌中的表达及意义

目的 观察热休克蛋白40（shock protein 40，HSP40）肝内胆管细胞癌组织、肝细胞癌组织及正常肝组织中的表达，探讨其在肝内胆管细胞癌发生中的作用，为肝内胆管细胞癌的早期诊断和治疗提供一定的指导意义。方法 应用免疫组化检测HSP40 在30例肝内胆管细胞癌组织、20例肝细胞癌组织和15例肝细胞癌旁组织中的表达情况；应用酶联免疫吸附实验（EL1SA）检测相应30例肝内胆管细胞癌患者、20例肝细胞癌患者和15例正常健康志愿者体内血清中HSP40的含量，ELISA试剂盒购自Cusabio公司。结果 HSP40在肝内胆管细胞癌患者中表达量为502.07±64.53，显著高于正常健康志愿者中表达量为105.31±27.00（P<0.02）；在肝细胞癌患者中表达量为412.00±53.41，并且HSP40在肝内胆管细胞癌患者中比肝细胞癌患者中明显较高。结论 HSP40在肝内胆管细胞癌中的高表达可能与与其癌变的发生和发展有着重要的关联，可作为肝内胆管细胞癌的辅助诊断指标之一及判断预后的重要指标。

Expression of Shock Protein 40 in Intrahepatic Bile Duct Carcinoma and Its Clinical Significance

Objective To observe the expression of heat shock protein (40 shock protein, 40 HSP40) in intrahepatic bile duct carcinoma, liver cancer tissue and normal liver tissue, to explore its role in intrahepatic bile duct carcinoma, for the early diagnosis and treatment of liver cancer provide certain guiding significance. Methods With immunohistochemical,we detected the expression of HSP4 in 30 cases of intrahepatic bile duct carcinoma tissue, 20 cases hepatocellular carcinoma tissues and 15 cases normal liver tissue. With Enzyme-linked immunosorbent assay, we detected the expression of HSP40 in serum of 30 cases of intrahepatic bile duct carcinoma, 20 cases hepatocellular carcinoma and 15 cases normal liver. Results HSP40 in patients with intrahepatic bile duct carcinoma was higher than that in normal healthy volunteers HSP40 in intrahepatic bile duct carcinoma in patients was significantly higher than that in patients with liver cancer. Conclusion HSP40 in intrahepatic bile duct carcinoma may be related to high expression which had significant correlation with its occurrence and development of canceration.It can be used as one of the auxiliary diagnosis indexes of intrahepatic cholangiocarcinoma, and one of the important indices for judging prognosis.