| HPLC法测定硫酸阿托品注射液的含量 |
| |
| 引用本文： | 李犁,牛慧云,邓雪英. HPLC法测定硫酸阿托品注射液的含量[J]. 医药世界, 2010, 0(8): 822-823 |
| |
| 作者姓名： | 李犁 牛慧云 邓雪英 |
| |
| 作者单位： | [1]包头医学院第三附属医院,内蒙古包头014030 [2]包头医学院,内蒙古包头014030包头医学院,内蒙古包头014030 |
| |
| 摘 要： | 目的建立HPLC法测定硫酸阿托品注射液含量的方法。方法采用C18（Alltech,4.6mm×250mm,5μm）色谱柱。流动相：乙腈-水-三乙胺（25：75：0.1,V/V）,流速1.0ml/min,紫外检测波长210nm,以非那西丁为内标。结果本法硫酸阿托品注射液在40～200μg/ml范围内线性关系良好（r=0.9978,高、中、低3种浓度回收率分别为101.2%、97.6%和102.3%,日内、日间RSD均〈5%）。结论本法操作简单,结果准确可靠,可用于硫酸阿托品注射液的质量控制。
|
| 关 键 词： | 色谱法,高压液相/方法 阿托品/分析 |
Determination of the Contents of Atropine Sulfate Injection by HPLC |
| |
| LI Li,NIU Hui-yun,DENG Xue-ying. Determination of the Contents of Atropine Sulfate Injection by HPLC[J]. Medicine World, 2010, 0(8): 822-823 |
| |
| Authors: | LI Li NIU Hui-yun DENG Xue-ying |
| |
| Affiliation: | .Third Affiliated Hospital of Baotou Medical College,Baotou 014030,China |
| |
| Abstract: | Objective To develop a HPLC internal standard method for determination of Atropine Sulfate Injection.Methods The C18 column （4.6 mm× 250 mm,5 μm）was used,acetonitrile-water-triethylamine （25：75：0.1,V /V） was used as mobile phase,flow rate：1.0 ml/min,The UV detection wavelength is 210 nm,Phenacetin Was selected as internal standard.Results The inearity was obtained from 40 to 200 μg/ml of Atropine Sulfate Injection（r =0.997 8,The average recoveries for Atropine Sulfate were 101.2%,97.6% and 102.3%,The RSD were less than 5%）.Conclusion The method is simple,accurate and reliable and can be used for quality control of atropine sulfate injection. |
| |
| Keywords: | Chromatography High Pressure Liquid/methods Atropine/analysis |
| 本文献已被 维普 等数据库收录！ |
|
