脆性组氨酸三联体和Ki67蛋白在卵巢上皮性肿瘤组织中的表达及其临床意义

目的:探讨脆性组氨酸三联体(fragile histidine triad,FHIT)和Ki67蛋白在卵巢上皮性肿瘤组织中的表达及临床意义。方法:采用免疫组化方法检测40例卵巢原发性上皮性癌、20例卵巢交界性肿瘤和20例卵巢良性腺瘤组织中FHIT和Ki67蛋白的表达。结果:卵巢上皮性癌中FHIT蛋白阳性率为65%(26/40),明显低于交界性肿瘤95%(19/20)和良性肿瘤100%(20/20),P值分别为0·012和0·002;而Ki67蛋白阳性率为65%(26/40),明显高于交界性肿瘤0(0/20)、良性肿瘤0(0/20),P值均为0·000。卵巢癌中浆液性癌FHIT蛋白表达率低于非浆液性癌(P=0.000),FHIT蛋白表达率与细胞分化程度(P=0·007)、FIGO分期(P=0·048)及淋巴结转移(P=0·018)相关,与术后残留灶大小(P=0·347)无关。卵巢癌中Ki67蛋白表达率与FIGO分期(P=0·007)、细胞分化程度(P=0·048)及淋巴结转移情况(P=0·041)相关,与病理类型(P=0·273)及残留灶大小(P=0·219)无关。卵巢癌中FHIT蛋白表达与Ki67蛋白的表达呈负相关,rs=-0·543,P=0·015。结论:FHIT基因表达下降,可能与卵巢癌发生和发展有关;联合检测FHIT和Ki67表达情况可能有助于判断卵巢癌患者的预后。

Study on expression and clinical significance of FHIT and Ki67 protein in primary epithelial ovarian tumor

OBJECTIVE: To investigate the expressions and correlation of fragile histidine triad and Ki67 protein in primary epithelial ovarian tumor.METHODS:Expressions of FHIT and Ki67 proteins in primary ovarian epithelial tumors(malignant n=40,borderline tumor n=20,benign adenoma n=20) were evaluated by the immunohistochemical stain. RESULTS: Expression of FHIT protein in primary epithelial ovarian cancers (65%, 26/40) was significantly lower than that in ovarian borderline tumors(95%,19/20),P=0.012, and ovarian benign adenoma (100%,20/20),P=0.002.Ki67 protein was detected positive in 65%(26/40) of the epithelial ovarian cancers,which was significantly higher than that in ovarian borderline tumor (0, 0 /20), and ovarian benign adenoma(0,0/20), P=0.000. Expression of FHIT protein in ovarian serous cancer was significantly lower than that in non-serous ones, P=0.000. Expression of FHIT protein was significantly correlated with the differentiated grade (P=0.007), FIGO stage (P=0.048) and lymph node metastasis, P=0.018. But was not correlated with the diameter of residual tumors, P=0.347. The present data demonstrated that the expression of Ki67 protein in epithelial ovarian cancer was significantly correlated with FIGO stage (P=0.007),the differentiated (P=0.048)and lymph node metastasis (P=0.041),but was not correlated with different histological classifications (P=0.273)and the volume of residual tumors, P=0.219.A significantly inverted relationship was observed between FHIT and Ki67 protein expressions in primary epithelial ovarian cancers(Spearman rank coefficience,r_s=-0.543,P=0.015).CONCLUSIONS:Decreasement of FHIT protein expression may be correlated with carcinogenesis and evolution of the epithelial ovarian cancer. The combinatory detection of FHIT and Ki67 protein expression may be helpful to decern the poorer prognostic patients.