巨细胞病毒感染与动脉粥样硬化关系的研究

近年,动脉粥样硬化(Atherosclerosis,As)发生的病毒病因学研究日益受到人们的关注.本研究通过应用ELISA、PCR及原位杂交等技术对45例As病人及22例正常者血清及血管组织配对标本检测了HCMV—sIgG、sIgM及HCMV IE、L基因片段,以及其在血管组织中的分布.结果显示:As组中HCMV—sIgG抗体水平显著高于对照组,sIgM仅见于As组.HCMV IE及L基因的阳性率也显著高于对照,并且发现HCMV DNA多见于内膜下肌层及中膜平滑肌的细胞核,强烈提示HCMV感染与As的发生密切相关.同时,本文还就HCMV—sIgG及sIgM与HCMV基因检出的关系及意义作了探讨,为As的临床早期诊断、早期预防及针对性治疗奠定了基础.

The study on the relationship between human cytomegalovirus infection and atherosclerosis development

Objective:Atherosclerosis (As) is one of the disease that seriously threaten human beings. Up to now, the causes of As are still a puzzle. Recently, increasing attention is being paid to the viral etiology of As. Human cy-tomegalovirus (HCMV) is thought to be a most possible etiological factor in As. Methods: Arterial vascular tissue specimens derived from 45 patients with As were studied for detection of human cytomegalovirus (HCMV) immediately early (IE) and later (L) gene fragments by polymerase chain reaction (PCR) and in situ hybridization Sera collected from the same patients were examined for HCMV specific IgG and IgM by enzyme - linked immunosorbent assay (ELISA). Twenty-two nomal arterial tissues and sera were used as controls. Results: The positive rate of HCMV L and IE gene fragments were significantly higher in As patients than those in controls. HCMV DNA were mainly observed in nucleus of endothelial cells、muscularis under intima and smooth muscle of media of As, rarely found in controls. Conclusion:1) there are some relationship between HCMV and As;2) artery itself may be the site of HCMV latency. Meanwhile, Higher levels of HCMV-slgG and slgM were found to be associated with As, indi-ating that a periodically actived latent infection or a continuously active infection is presented in As patients.