大鼠脊髓神经肽Y、神经降压肽免疫反应神经元向结合臂旁核的投射

用HRP逆行束路追踪法结合免疫细胞化学双标记技术,研究了大鼠颈、胸、腰、骶各段脊髓神经肽Y(NPY)、神经降压肽(NT)免疫反应神经元向结合臂旁核的投射。HRP逆行标记细胞分布于两侧脊髓灰质第Ⅰ、Ⅱ、Ⅳ、Ⅴ、Ⅶ层、中央管背侧灰质连合及外侧颈核、外侧脊髓核中,以对侧为主。NPY免疫反应阳性神经元分布于两侧后角第Ⅰ层、第Ⅱ层浅部、外侧颈核、外侧脊髓核及骶髓后连合核中。NT免疫阳性神经元分布于两侧后角第Ⅰ层及第Ⅱ层中。在后角第Ⅰ层、外侧颈核和外侧脊髓核中,可观察到部分NPY-HRP双标记神经元;在后角第Ⅰ层可观察到部分NT-HRP双标记神经元。本研究结果提示,NPY和NT神经元可能参与脊髓-结合臂旁核的痛觉传递。

SPINAL PROJECTION OF NEUROPEPTIDE Y-AND NEUROTENSIN-IMMUNOREACTIVE NEURONS TO THE PARABRACHIAL NUCLEUS IN THE RAT

In this study a combination of HRP retrograde tract tracing and immunocytochemistry was employed to investigate the afferent projections of neuropeptide Y(NPY) -and neurotensin (NT)-immunoreactive(ir) neurons in the cervical, thoracic, lumbar and sacral spinal segments to the parabrachial nucleus in the rat. It was demonstrated that the retrogradely labeled neurons were scattered in bilateral laminae I, II, IV, V, VII, gray matter commissure dorsal to the central canal, lateral cervical nucleus and lateral spinal nucleus. The NPY-ir neurons were situated in bilateral lamina I, superficial portion of the lamina II, lateral cervical nucleus and lateral spinal nucleus. The NT-ir cells were seen in bilateral lamina I and lamina II of dorsal horn. In lamina I, lateral cervical nucleus and lateral spinal nucleus a few of NPY-HRP double labelled neurons could be observed. Individuals of NT-HRP double labeled neurons were identified only in lamina I. The results of this study suggest that NPY and NT neurons probably involved in the nociceptive transmission of the spino-parabrachial pathway.