Tracheal growth after resection and anastomosis in puppies |
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| Authors: | H Blanchard P Brochu A L Bensoussan G Lagacé A H Khan |
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| Affiliation: | 1. Department of Plastic and Reconstructive Surgery, Shanghai 9th People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai Key Laboratory of Tissue Engineering, Shanghai, PR China;2. National Tissue Engineering Center of China, Shanghai, PR China;3. Institute of Dermatology, Chinese Academy of Medical Sciences and Peking Union Medical College, Nanjing, PR China;4. Research Institute of Plastic Surgery, Plastic Surgery Hospital, Wei Fang Medical College, Weifang, Shandong, PR China;5. Department of Comparative Pathobiology, College of Veterinary Medicine, Purdue University, West Lafayette, IN, 47907, USA;6. Department of Thoracic Surgery, Shanghai Pulmonary Hospital, Tongji University School of Medicine, Shanghai, PR China;1. Department of Thoracic Surgery, Tangdu Hospital, Air Force Medical University, Xi‵an, Shaanxi, 710038, China;2. Department of Cardiothoracic Surgery, Central Theater Command General Hospital of Chinese People''s Liberation Army, Wuhan, Hubei, 430070, China;3. Department of General Surgery, The 991st Hospital of the Chinese People''s Liberation Army Joint Logistic Support Force, Xiangyang, Hubei, 441000, China;4. State Key Laboratory for Manufacturing Systems Engineering, Xi''an Jiaotong University, Xi''an, Shaanxi, 710049, China;5. Department of Cardiothoracic Surgery, The 980th Hospital of the Chinese People''s Liberation Army Joint Logistic Support Force, Shijiazhuang, Hebei, 052460, China;6. State Key Laboratory for Mechanical Behavior of Materials, And Frontier Institute of Science and Technology, Xi''an Jiaotong University, Xi''an, Shaanxi, 710049, China;7. Institute of Medical Science, Temerty Faculty of Medicine, University of Toronto, Toronto, Ontario, Canada;8. Latner Thoracic Surgery Research Laboratories, Toronto General Hospital Research Institute, University Health Network, Toronto, Ontario, Canada;9. Department of Ophthalmology, Tangdu Hospital, Air Force Medical University, Xi‵an, Shaanxi, 710038, China;1. Department of Cardiothoracic Surgery, Shanghai Children''s Medical Center, Shanghai Jiao Tong University School of Medicine, 1678 Dongfang Road, Shanghai 200127, People''s Republic of China;2. Department of Pediatric Cardiac Surgery, National Center for Cardiovascular Disease and Fuwai Hospital, Chinese Academy of Medical Sciences, Peking Union Medical College, 167 Beilishi Road, Xicheng, Beijing 100037, People''s Republic of China;3. Department of Plastic and Reconstrucive Surgery, Shanghai 9th People’s Hospital, 639 Zhi Zao Ju Road, Shanghai 200011, People''s Republic of China;4. Institute of Pediatric Translational Medicine, Shanghai Children’s Medical Center, Shanghai Jiaotong University School of Medicine, 1678 Dong Fang Road, Shanghai 200127, People''s Republic of China |
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| Abstract: | Tracheal morphology, morphometric changes, and growth and histologic changes were studied in puppies submitted to tracheal resection and anastomosis. Fifteen mongrel puppies about 12 weeks old and weighing on an average 5.5 kg were operated under general anesthesia using fluothane. A median cervicotomy incision was made in ten puppies (experimental group, EG) and the proximal 14 tracheal rings were resected (average length 5.08 cm or about 35% to 38% of total tracheal length). One layer anastomosis was done using vicryl 4.0 maintaining the average tension of 1,450 g. Five puppies (control group, CG) were submitted to tracheal transection and anastomosis and the following parameters were studied. Tracheal morphology the trachea of the EG was a rounded triangle whereas in the CG it was oval in shape, there was increase in the intercartilageneous spaces in the EG, no granulation tissue was present, two mucous webs were seen in the EG and one in the CG. Morphometric changes average tracheal length EG 13 cm, CG 17.7 cm, intercartilagenous space EG 3.08 mm, CG 1.3 mm, intercricothyroid space EG 1.2 cm, CG 0.53 cm, sagittal and transverse tracheal thickness at the anastomosis EG 2.6 and 3.3 mm, CG 2 and 1.5 mm, sagittal and transverse diameter reduced on an average 2 mm in EG. Histology Moderate fibrosis was found at the level of anastomosis with no modification of chondrocytes at the cartilagenous rings in the EG. Even with high anastomotic tension, the dogs had normal tracheal growth without stenosis; the sagittal and transverse growth at the anastomosis in the EG was 90% and 85%, respectively, when compared with the CG. |
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