| HPLC法测定双黄消炎片中盐酸小檗碱的含量 |
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| 引用本文： | 周家才,梁玉梅,邱震.HPLC法测定双黄消炎片中盐酸小檗碱的含量[J].安徽医药,2009,13(3):267-269. |
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| 作者姓名： | 周家才 梁玉梅 邱震 |
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| 作者单位： | 南京医药合肥天星有限公司,安徽合肥,230061;南京医药合肥天星有限公司,安徽合肥,230061;南京医药合肥天星有限公司,安徽合肥,230061 |
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| 摘 要： | 目的采用高效液相色谱法测定双黄？肖炎片中盐酸小檗碱的含量。方法Kromasil C18色谱柱（250mm×4．6mm，5μm）；乙腈-0．05mol·L^-1磷酸二氢钾溶液（25：75）为流动相，流速为1．0ml·min^-1；检测波长为347am。结果盐酸小檗碱线性范围为9．2。146．4mg·L^-1（r=0．9999，n=5），平均回收率为99．93％，RSD=0．60％（n=6）。结论该方法准确、简便，可用于该制剂的质量控制。
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| 关 键 词： | 高效液相色谱法 盐酸小檗碱 双黄消炎片 |
Determination of berberine in shuanghuangxiaoyan tablets by HPLC |
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| ZHOU Jia-cai,LIANG Yu-mei,QIU Zhen.Determination of berberine in shuanghuangxiaoyan tablets by HPLC[J].Anhui Medical and Pharmaceutical Journal,2009,13(3):267-269. |
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| Authors: | ZHOU Jia-cai LIANG Yu-mei QIU Zhen |
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| Institution: | ZHOU Jia-cai,LIANG Yu-mei,QIU Zhen(Nanjing Medical Hefei Tianxing Co,Ltd,Hefei 230061) |
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| Abstract: | Aim To establish an HPLC method for determining berberine in shuanghuangxiaoyan tablets. Method C18 column（250 mm × 4.6 mm,5 μm） was used and the mobile phase was composed of acetonitrile-0.05 mol · L ^- 1KDP （ 25 ： 75 ）. The flow rate was 1.0 ml · min ^- 1. The detection wavelength was at 347 nm. Result The linear range of berberine was from 9.2 to 146.4 mg· L ^- 1 （ r = 0. 999 9, n = 5 ） and the average recovery was 99.93 % with an RSD of 0.60% （ n = 6）. Conclusion The method is accurate and simple for quality control of the preparation. |
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| Keywords: | HPLC berberine shuanghuangxiaoyan tablets |
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