| HPLC法同时测定葛根芩连片中黄芩苷和葛根素的含量 |
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| 引用本文： | 刘利敏,胡译方.HPLC法同时测定葛根芩连片中黄芩苷和葛根素的含量[J].安徽医药,2012,16(11):1598-1600. |
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| 作者姓名： | 刘利敏 胡译方 |
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| 作者单位： | 广西省桂林市食品药品检验所;中国人民解放军第181医院,广西,桂林,541002 |
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| 摘 要： | 目的建立HPLC法同时测定葛根芩连片中黄芩苷和葛根素的含量。方法用Agilent Eclipse XDB—C18柱（5μm，4．6mm×250mm）；流动相A为甲醇，流动相B为0．2％磷酸溶液，二元梯度洗脱，检测波长：277nm；流速：1ml·min-1；柱温：30℃。结果黄芩苷进样量在0．1794～3．5870μg范围内，葛根素进样量在0．1783～3．5650μg范围内线性关系良好，平均加样回收率分别为98．7％和98．8％。结论该法灵敏、简便、准确，可用于葛根芩连片质量控制。关键词：高效液相色谱法；葛根芩连片；梯度洗脱；黄芩苷；葛根素
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| 关 键 词： | 高效液相色谱法 葛根芩连片 梯度洗脱 黄芩苷 葛根素 |
HPLC determination of baicalin and puerarin in gegenqinlian tablets |
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| LIU Li-min,HU YI-fang.HPLC determination of baicalin and puerarin in gegenqinlian tablets[J].Anhui Medical and Pharmaceutical Journal,2012,16(11):1598-1600. |
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| Authors: | LIU Li-min HU YI-fang |
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| Institution: | 1.Guilin Institute for Food and Drug Control;2.181st Hospital of the Chinese People’s Liberation Army,Guilin 541002,China) |
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| Abstract: | Objective To establish a method for determination of baicalin and puerarin in gegenqinlian tablets by HPLC.Methods The Agilent Eclipse XDB-C18 column(5 μm,4.6 mm×250 mm) was used.The mobile phase A consisted of methanol and the mobile phase B 0.2% phosphoric acid solution.The flow rate was 1.0 ml ·min-1.The detection wavelength was 277 nm and the column temperature was 30℃.Results The linear range of baicalin was 0.179 4~3.587 0 μg and range of puerain was 0.178 3~3.565 0 μg.The average recovery rate was 98.7% and 98.8%.Conclusion The method is convenient,accurate,which can be used for determination of baicalin and puerain in gegenqinlian tablets. |
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| Keywords: | HPLC gradient elution gegenqinlian tablets baicalin puerarin |
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