Biological properties associated with the enhanced lung-colonizing potential in a B16 murine melanoma line grown in a medium conditioned by syngeneic Corynebacterium parvum-elicited macrophages |
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Authors: | Lido Calorini Antonella Mannini Francesca Bianchini Gabriele Mugnai Manuela Balzi Aldo Becciolini Salvatore Ruggieri |
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Institution: | (1) Department of Experimental Pathology and Oncology, Sweden;(2) Department of Clinical Physiopathology, Radiation Biology Laboratory, University of Florence, Florence, Italy |
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Abstract: | A previous study by our laboratory showed that the peritoneal murine Corynebacterium parvum-elicited macrophages released into their growth medium an activity which enhanced the ability of B16-F10 melanoma cells to
form experimental metastases in the lung of syngeneic mice. In the present study, we used a clone of B16-F10 line (F10-M3
cells) to investigate whether the increase in lung-colonizing potential due to the pro-clonogenic activity released by C. parvum-elicited macrophages was associated with biological properties characteristic of a metastatic phenotype. We have found that
the pulmonary retention, growth rate in lung parenchyma, invasiveness through Matrigel, adhesiveness to IL-1-activated endothelium
and MHC class I expression were increased in F10-M3 cells stimulated by the macrophage pro-clonogenic activity. By using an
in vitro experimental protocol, the enhancement of lung-colonizing potential in the stimulated melanoma cells turned out to be a transient
phenomenon as was the increase of invasiveness through Matrigel and the higher expression of MHC class I antigens. In conclusion,
the melanoma cells stimulated by the pro-clonogenic activity released by C. parvum-elicited macrophages showed changes in biological parameters which are relevant to metastatic diffusion. These changes appeared
as a temporary phenomenon which sustains the view that the metastatic phenotype represents a transient biological character
influenced by host factors.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | B16-F10 cells Corynebacterium parvum-elicited macrophages adhesiveness invasiveness MHC class I antigens |
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