骨肉瘤组织中雌、孕激素受体的表达及临床意义

目的探讨骨肉瘤组织中雌激素受体（ＥＲ）、孕激素受体（ＰＲ）的表达及其与临床特征的关系。方法应用免疫组化改良ＳＰ方法对６５例骨肉瘤蜡块组织进行了ＥＲ、ＰＲ检测。结合临床特点进行统计学分析。所有病人术前均未接受内分泌治疗。结果骨肉瘤中ＥＲ表达的阳性率为４０％（２６／６５），ＰＲ表达的阳性率为３２３％（２１／６５）；临床无转移，存活时间长之骨肉瘤中ＥＲ、ＰＲ表达的阳性率高于临床有转移、存活时间短者，差异有显著性意义（Ｐ＜００５）；不同性别、年龄、发病部位的骨肉瘤中ＥＲ、ＰＲ表达的阳性率无显著性差异（Ｐ＞００５）。结论骨肉瘤中存在ＥＲ、ＰＲ，ＥＲ、ＰＲ的检测可提示为骨肉瘤生物学行为，对病人预后的评估及探讨骨肉瘤内分泌治疗提供重要的理论依据。

Expression and clinical significance of estrogen receptor and progesterone receptor detection in human osteosarcoma

Objective To investigate the expression of ER, PR in osteosarcoma and the relationship between ER,PR and clinical characteristics of osteosarcoma. Methods ER and PR were detected by streptavidin/peroxidase(SP) method immunohistochemically using paraffin embeded tissues from 65 cases of osteosarcoma. Patients consisted of 40 men and 25 women with average age of 22 years, no patient was treated by endocrinotherapy before operation. Results The positive rate of ER,PR in osteosarcoma was 40%(26/65), 32.3%(21/65), respectively. The positive particles were distributed in cytoplasm and nucleus. Higher positive rates of ER,PR were observed in patients without metastasis and longer survival time than those with metastasis and shorter survival time. The differences were statistically signiticant (P<0.05).There were no relations between the expression of ER,PR and the sex,age and tumor site.Conclusion ER,PR exist in osteosarcoma and their positive tests are important in evaluating the clinical behavior and prognosis of osteosarcoma.