实验性脑创伤后脑损害生化标志物水平的动态变化

目的探讨脑创伤后脑损害生化标志物（NSE和S100B蛋白）的动态变化规律及其在血清与脑脊液中的相关性。方法采用改良Feneey法制作右顶局灶性脑损伤模型。选择60只SD大鼠分为假手术组和伤后1h，3h，6h，12h和24h组（各10只）。各组在相应时间点经枕大池和心脏穿刺抽取脑脊液和血液标本，测定NSE和S100B蛋白含量。结果与假手术组相比，手术组于伤后1h的NSE水平差异无显著性（P〉0．05），3h开始升高，6h达高峰，12h下降，24h降至正常；而S100B蛋白在伤后1h就明显增高，并持续升高至伤后6h，12h～24h有所下降，但仍高于假手术组（P〈0．05）。脑脊液NSE和S100B蛋白水平与其血清水平呈直线相关（NSE：r=0.78，P〈0．05；S100B：r=0．65，P〈0．05）。结论联合监测血NSE和S100B蛋白含量的变化能真实准确的反映出脑损害的情况，具有简单、方便和快速的优点。

Signifcance and Changes of Neurobiochemical Markers (Nse and S100b) of Brain Damage after Experimental Brain Trauma

Objective To investigate the dynamic changes of neurobiochemical markers(NSE and S100B) of brain damage and the correlation between serum levels and CSF levels of them after experimental brain trauma.Methods Sixty SD rats underwent a severe cortical impact injury(2.5mm deformation)according to modified Feeney method.Blood and CSF samples were drawn at 1h,3h,6h,12h and 24h after trauma as well as in sham operated animals.Results We found a time-dependent release of NSE in serum and CSF after trauma;the highest NSE value was detected at 1h after trauma.The highest S100B protein level was measured 1h after trauma,whereas S100B protein serum and CSF levels showed an increase but no time dependent release.There was a positive correlation between serum and CSF levels of neurobiochemical markers of brain damage.Conclusions Combined detecting serum NSE and S100B protein serum levels can accurated quickly reflect brain damage degree and range,which is a simple convenient method.