不同产地人参对脾虚小鼠T淋巴细胞功能影响的实验研究

目的]探讨辽宁、吉林、朝鲜三产地人参对小鼠T淋巴细胞功能的影响。方法]采用健康SCID小鼠80只随机分为空白对照组、正常人参对照组(三组)及模型组、脾虚人参治疗组(三组)。模型组及治疗组以100%番泻叶浸液灌服,建立脾虚证模型。造模成功后,脾虚人参治疗组和正常人参对照组分别以三产地人参煎剂灌胃,5g/Kg/d/只,连续给药2周。2周后通过迟发型超敏反应(DTH)观察小鼠足趾DTH程度及E花环形成实验检测其T淋巴细胞功能状态。结果]模型组小鼠DTH程度与E花环形成百分率均低于空白对照组(P<0.01),经三地人参治疗后两项指标均较模型组升高(P<0.01),三地人参治疗组与空白对照组间无差异,三者间疗效比较也无显著差异(P>0.05);正常三地人参对照组与空白对照组间无差异(P>0.05),三组之间比较也无统计学意义(P>0.05)。结论]脾虚可导致小鼠DTH程度及E花环形成百分率降低;三地人参均能提高脾虚小鼠DTH程度及E花环形成百分率,并能使其达到正常水平。证明三地人参对正常小鼠的T细胞功能没有明显影响,三地人参对脾虚证小鼠T淋巴细胞功能的调节基本相同。

The Experimental Study To T- Lymphocyte Of The Splenasthenic Syndrome Mice Caused By Different Species Ginseng

Objective] to explore the Liaoning, Jilin, Korea ginseng of mice industry to T lymphocyte function. Methods] 80 SCID mice divided into 8 control group, blank , 3groups feed with panax ginseng water , model group and 3 model group feed with panax ginseng water, establish the spleen deficiency model through Gastric lavage with 100% senna water. After the success of pixu ginseng treatment group and control group by failing to normal ginseng respectively to ginseng decoction lavage 5g/kg/d, only for medicine, last 2 weeks. Two weeks later detective the hypersensitivity reactions （DTH） mice DTH toes and E -wreath to test its T - lymphocyte function. Results ] The model group of mice DTH and E - rosette formation are lower than blank control group（ P 〈 0.01 ） , the both indexes among the three origins of ginseng after treatment were upper than model group（ P 〈 0.01 ） ,but no significant difference comparison between thouse three groups ; Compared with the ginseng control group and control group have slight change, but was not statistically significant （P 〉 0. 05）, and between the three group is also no statistical significance （P 〉 0.05 ）. Conclusion] Spleen defi- ciency can cause the mouse DTH and E - rosette form percentage reduce ; Three origin of ginseng can restore splenasthenic syndrome mice DTH and E -rosette form percentage, explain that ginseng can adjust splenas- thenic syndrome and make the T - lymphocyte function reduction ; Comparison between the three origin gin- seng, Splenasthenic syndrome mice to adjust T - lymphocyte function basically the same;Three of the origins of ginseng for normal mice T -lymphocyte function have no obvious affections.