莪红注射液对大鼠心肌缺血再灌注保护作用的研究

目的 :探讨莪红注射液保护心肌缺血再灌注损伤的作用机制。方法 :将 2 8只Wistar大鼠随机分为 4组 :空白对照组 ( 6只 )、假手术组 ( 6只 )、莪红注射液小剂量组 ( 8只 )、莪红注射液大剂量组 ( 8只 )。采用结扎冠状动脉前降支方法造成大鼠急性心肌缺血再灌注模型 ,将心肌组织制成冰冻切片 ,与大鼠T淋巴细胞悬液共温后 ,常规HE染色和显微镜观察 ,求出淋巴细胞与血管壁的特异性黏附率。结果 :心肌缺血再灌注后各时间点的T淋巴细胞黏附率均明显高于假手术组(P <0 .0 1) ,莪红注射液组T淋巴细胞黏附率明显下降 (P <0 .0 5 )。结论 :莪红注射液抗白细胞与内皮细胞黏附可能是活血化瘀中药治疗急性心肌缺血再灌注损伤的有效途径之一。

Experimental Study on Proctective Effect of Injection Ehong on Cardiac Ischemia-Reperfusion in Rats

Objective:To investigate the proctective effect mechanism of injectio Ehong on cardiac ischemia-reperfusion damage in rats. Methods:Twenty-eight wistar rats were randomly divided into 4 groups: normal controls group(n=6), sham operation group(n=6), small dose of injectio Ehong group(n=8),high dose of injectio Ehong group(n=8). Coronary artery were ligated in rats.Cardiac tissue were made into frozen section and mixed with T lymphocyte of rats, then it were observed under microscope after HE dyeing, T lymphocyte specific adherence rate were calculated. Results:T lymphocyte specific adherence rate in cardiac ischemia-reperfusion groups were higher than that in sham operation group (P<0.01), T lymphocyte specific adherence rate in injectio Ehong groups were decreased obviously. Conclusion:Injectio Ehong could decrease T lymphocyte specific adherence rate in rats with cardiac ischemia-reperfusion damage, it may be one of mechanism underlying the proctective effect of the traditional chinese medicine on cardiac ischemia-reperfusion damage.