骨化三醇对单侧输尿管梗阻大鼠肾小管上皮细胞转分化影响的研究

目的观察幼年大鼠单侧输尿管梗阻(UUO)肾组织中α-肌动蛋白(α-SMA)、E-钙黏蛋白(E-Cad)、转化生长因子-β1(TGF-β1)、骨形态发生蛋白-7(BMP-7)mRNA的表达,探讨骨化三醇对肾小管上皮细胞转分化(EMT)干预效应及机制。方法 54只雄性SD大鼠随机分为3组:假手术组(A组)、UUO模型组(B组)及UUO模型干预组(C组)各18只,灌胃满3、7、14d取肾。实时荧光定量聚合酶链反应(RT-PCR)法检测各组α-SMA、E-Cad、TGF-β1、BMP-7mRNA表达水平。结果与A组相比,B组E-Cad、BMP-7mRNA表达明显下降(P<0.01),α-SMA、TGF-β1mRNA表达显著增高(P<0.01);C组与B组相比,E-Cad、BMP-7mRNA表达明显上调(P<0.01),α-SMA、TGF-β1mRNA表达明显下降(P<0.01)。结论骨化三醇对UUO模型大鼠肾小管上皮细胞转分化有抑制作用,这种抑制作用可能与下调TGF-β的表达及上调BMP-7的表达有关。

Effects of calcitriol on tubular epithelial myofibroblast transdifferentiation in rats with unilateral ureteral obstruction

Objective To determine the effects and potential mechanisms of calcitriol on tubular epithelial my- ofibroblast transdifferentiation （EMT） in young rats with unilateral ureteral obstruction （UUO）, based on the expression of renal tubulointerstitial or-smooth muscle actin （α-SMA）, E-cadherin （E-Cad）, transforming growth factor-β1 （TGF-β1） and bone morphogenetic protein-7 （BMP-7） mRNA. Methods Fifty-four male Sprague-Dawley rats were randomly al- located into Sham group （group A, n=18）, UUO model group （group B, n=18） and UUO treatment group （group C, n= 18）. The renal tissue was biopsied at days 3, 7 and 14 for subsequent assays of α-SMA, E-Cad, TGF-β1 and BMP-7 mRNA expression by using real-time fluorescence quantitative polymerase chain reaction （RT-PCR）. Results Com- pared with group A, significantly attenuated E-Cad and BMP-7 mRNA expression yet markedly increased α-SMA and TGF-β1 mRNA was noted in group B （all P〈0.01）. Group C was characterized by considerably up-regulated E-Cad and BMP-7 mRNA expression yet down-regulated expression of α-SMA and TGF-β1 mRNA when compared with group B （all P〈0.01）. Conclusion The fact that calcitriol suppresses EMT in rats with UUO may be associated with down- regulation of TGF-β1 mRNA expression and up-regulated expression of BMP-7 mRNA.