| 珠子草总多酚含量研究 |
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| 引用本文： | 李彩东. 珠子草总多酚含量研究[J]. 中国药物与临床, 2013, 13(4): 440-442 |
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| 作者姓名： | 李彩东 |
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| 作者单位： | 730046,兰州市第二人民医院制剂中心 |
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| 基金项目： | 兰州市科技发展计划项目 |
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| 摘 要： | 目的建立珠子草总多酚含量测定方法,并对珠子草中总多酚含量进行测定。方法采用普鲁士蓝法使酚类物质配位显色,紫外分光光度法测定。结果在0.5274～1.5821μg/ml范围内,没食子酸浓度与吸光度呈良好的线性关系。平均加样回收率99.26%,相对标准偏差(RSD)1.46%。结论分析方法操作简便,精密度及准确度高,重现性及稳定性好,可用于珠子草药材中多酚含量的测定。
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| 关 键 词： | 分光光度法-紫外线 普鲁士蓝法反应 珠子草 总多酚 |
Determination of total polyphenol in Phyllanthus niruri Linn |
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| LI Cai-dong. Determination of total polyphenol in Phyllanthus niruri Linn[J]. Chinese Remedies & Clinics, 2013, 13(4): 440-442 |
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| Authors: | LI Cai-dong |
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| Affiliation: | LI Cai-dong. (Medical Preparations Center, the Second People＇s Hospital of Lanzhou, Gansu 730046, China) |
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| Abstract: | Objective To establish the methodology for determination of total polyphenol in Phyllanthus niruri Linn. Methods Prussian blue was employed for assessment of polyphenol coloration by ultraviolet spectrophotome- try. Results A linear correlation between density and absorption of galic acid solution was noted at the concentration of 0.527 4~1.582 1 Ixg/ml, with a mean recovery of 99.26% and RSD of 1.46%. Conclusion The analytic approach using Prussian blue is characterized by convenience, high precision and accuracy, preferable repeatability and stabili- ty and may be feasible for assessment of galic acid in Phyllanthus niruri Linn. |
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| Keywords: | Spectrophotometry ultraviolet Prussian blue reaction Phyllanthus niruri Linn Total polyphe- nol |
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