| (-)-S·R-蝙蝠葛苏林碱对N-甲基-D-天门冬氨酸引起大鼠皮层神经元损伤的保护作用 |
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| 引用本文： | 刘景根,皋聪,李瑞,刘国卿,王金唏. (-)-S·R-蝙蝠葛苏林碱对N-甲基-D-天门冬氨酸引起大鼠皮层神经元损伤的保护作用[J]. 中国药理学与毒理学杂志, 1998, 0(4) |
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| 作者姓名： | 刘景根 皋聪 李瑞 刘国卿 王金唏 |
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| 作者单位： | 中国药科大学药理学教研室，南京大学配位化学国家重点实验室 |
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| 摘 要： | 蝙蝠葛苏林碱（ｄａｕｒｉｓｏｌｉｎｅ，Ｄａｕ）是从防己科植物蝙蝠葛根茎中提取的生物碱，（－）－Ｓ·Ｒ－Ｄａｕ是其人工合成非对映异构体．研究表明它的药理活性比天然品强很多［１］．我们最近报道（－）－Ｓ·Ｒ－Ｄａｕ能抑制谷氨酸（Ｇｌｕ）对原代培养的大鼠皮...
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| 关 键 词： | 神经元；大脑皮层；钙；N-甲基-D-天冬氨酸；蝙蝠葛苏林碱 |
Protective effect of (-) S·R daurisoline on N methyl D aspartate induced neuronal injury in rat primary cortical cultures |
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| LIU Jing Gen,GAO Cong,LI Rei,LIU Guo Qing,WANG Jing Xi. Protective effect of (-) S·R daurisoline on N methyl D aspartate induced neuronal injury in rat primary cortical cultures[J]. Chinese Journal of Pharmacology and Toxicology, 1998, 0(4) |
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| Authors: | LIU Jing Gen GAO Cong LI Rei LIU Guo Qing WANG Jing Xi |
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| Affiliation: | LIU Jing Gen,GAO Cong,LI Rei,LIU Guo Qing,WANG Jing Xi 2 |
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| Abstract: | Cultured neurons obtained from rat cortex were used to examine the protective effect of (-) S·R daurisoline (Dau) against N methyl D aspartate (NMDA) induced neurotoxicity. Cell viability was estimated by using trypan blue dye exclusion and MTT assay.Intracellular free Ca 2+ concentration ([Ca 2+ ] i ) was measured by using AR CM MIC cation measurement system with Fura 2/AM as Ca 2+ senstive fluorecent indicator. It was found that Dau could significantly inhibit NMDA induced neurotoxicity and block NMDA elicited increase of [Ca 2+ ] i in concentration dependent manner with IC 50 value of 7.1 and 6.4 μmol·L 1 respectively. It is suggested that Dau has a significant protection against NMDA induced neurotoxicity,the mechanism underlying the protection might be related to its antagonism against Ca 2+ influx into cells via NMDA mediated ligand gated ion channels. |
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| Keywords: | neurons cerebral cortex calcium N methyl D aspartate daurisoline |
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