胆管癌中微卫星不稳定的变化

目的:探讨胆管癌中微卫星不稳定(MSI)的变化。方法:肝外胆管癌标本70例、肝外胆管结石胆管组织40例,饱和氯化钠法提取组织DNA,PCR-SSCP变性聚丙烯酰胺凝胶电泳检测MSI,根据规定标准判定microsatellite stable(MSS),low-frequency MSI(MSI-L)和high-frequency MSI(MSI-H),计算总MSI率。结果:在肝外胆管结石胆管组织、胆管癌(无转移)、胆管癌(区域淋巴结或局部/远处转移)中MSI率分别为5.0%、21.1%、37.5%,高中分化腺癌及低分化腺癌中MSI率分别为32.6%、22.2%。结论:MSI与胆管癌的发生及浸润转移有关。

Changes of microsatellite instability in cholangiocarcinoma

Objective:To investigate the changes of microsatellite instability(MSI) in cholangiocarcinoma.Methods:We’ve got 70 extrahepatic cholangiocarcinoma samples and 40 bile duct tissue samples of extrahepatic cholangiolithiasis;then we extracted DNA of the tissue by saturated sodium chloride method,detected MSI through PCR-SSCP denaturing polyacrylamide gel electrophoresis,determined microsatellite stable(MSS),low-frequency MSI(MSI-L) and high-frequency MSI(MSI-H) according to required standards and at last calculated total MSI rate.Results:The corresponding MSI rates were 5.0%,21.1% and 37.5% in bile duct tissue of extrahepatic cholangiolithiasis,cholangiocarcinoma(without metastasis) and cholangiocarcinoma(regional lymph nodes or partial/distant metastasis),as for the high or middle differentiated adenocarcinoma and the low differentiated adenocarcinoma,corresponding MSI rates were 32.6% and 22.2%.Conclusion:MSI has a relationship with the occurrence and metastasis of cholangiocarcinoma.