宫颈癌中p16基因启动子异常甲基化的检测

目的:探讨宫颈癌组织及外周血浆中p16基因启动子异常甲基化的状况及其在宫颈癌诊断中的价值.方法:用甲基化特异性PCR技术对宫颈癌组织,正常宫颈组织及相对应血浆中p16基因进行甲基化的检测.结果:45例宫颈癌组织中p16基因异常甲基化率为33.3％(15/45),相对应血浆中p16基因甲基化的检出率为20％(9/45),而正常对照组织未检出甲基化.血浆中甲基化的改变与宫颈癌组织甲基化状态显著相关(P＜0.05).p16基因甲基化发生率与年龄、病理分级、临床分期之间无统计学相关性(P＞0.05).结论:p16基因CpG岛甲基化是宫颈癌发生的高频事件,其甲基化检测在宫颈癌早期诊断中有一定的应用价值.

Detection of p16 gene promoter methylation in cervical carcinoma patients

Objective:To evaluate the status and diagnostic value of hypermethylated p16 gene promoter in cancer tissue and peripheral plasma of cervical cancer patients.Methods:We analyzed the hypermethylation status of p16 gene in 45 cases of cervical carcinoma tissues and corresponding blood plasma,10 cases of normal cervical tissues by methylation-specific PCR.Results: Methylation rates of p16 gene were 33.3% and 20.0%,respectively,in cancer tissue and corresponding peripheral blood plasma,which were significantly coincident and higher in tumors compared with no hypermethylation being present in normal controls(P<0.05).The aberrant methylation of p16 gene in cancer tissues had no correlation with the patients’ clinicopathological features such as age,tumor size,pathological grade,staging(P>0.05).Conclusion: The aberrant methylation of p16 gene is prevalent in the plasma and tissue samples from cervical cancer patients,detection of aberant methylation change may contribute to application value in early diagnosis of cervical carcinoma.