吡咯烷二硫氨基甲酸酯对犬体外循环中红细胞的保护作用

目的研究犬体外循环(CPB)中吡咯烷二硫氨基甲酸酯(PDTC)对红细胞的保护作用。方法将12只犬随机分为对照组(n=6)和PDTC组(n=6),建立CPB模型。PDTC组于CPB前静脉注射PDTC30mg/kg,对照组于CPB前静脉注射等量生理盐水。分别于CPB前、阻断30min、阻断60min、开放30min和开放60min时测定血浆白细胞介素(IL)-lβ、IL-8、游离血红蛋白(F-HB)、丙二醛(MDA)、红细胞三磷酸腺苷(E-ATP)和红细胞超氧化物歧化酶(E-SOD)水平。结果阻断后各时间点对照组的血浆IL-lβ和IL-8水平均显著高于CPB前(P<0·01),PDTC组开放后血浆IL-lβ和IL-8水平显著高于CPB前(P<0·05,P<0·01);两组血浆MDA和F-HB水平均显著高于CPB前(P<0·01);两组E-ATP和E-SOD水平均显著低于CPB前(P<0·01)。PDTC组CPB后E-ATP水平和E-SOD活性显著高于对照组(P<0·01),而血浆IL-lβ、IL-8、MDA含量和F-HB浓度均显著低于对照组(P<0·01)。结论PDTC通过减轻CPB期间脂质过氧化反应和炎性反应对红细胞起到一定的保护作用。

Protective effect of pyrrolidine dithiocarbamate on erythrocytes during canine cardiopulmonary bypass

OBJECTIVE: To investigate the protective effect of pyrrolidine dithiocarbamate (PDTC) on erythrocytes during canine cardiopulmonary bypass (CPB). METHODS: Twelve adult healthy dogs undergoing CPB were randomly divided into the control group (n = 6) and the PDTC group (n = 6). In the PDTC group, PDTC 30 mg/kg was administered intravenously before CPB. Dogs in the control group was intravenously administering with normal saline. The levels of interleukin (IL)-1beta, IL-8, malondiadehyde (MDA), free hemoglobin (F-HB) in plasma, erythrocyte adenosine triphosphate (E-ATP), and erythrocyte superoxide dismutase (E-SOD) were determined before CPB, 30 and 60 minutes after aortic cross-clamping (AC), and 30 and 60 minutes after declamping (DC). RESULTS: In the control group, plasma levels of IL-1beta and IL-8 significantly increased after CPB (P < 0.01). In the PDTC group, plasma levels of IL-1beta and IL-8 significantly increased after CPB (P < 0.05, P < 0.01). Plasma levels of MDA and F-HB significantly increased (P < 0.01) and the E-ATP level and E-SOD activity significantly decreased after CPB (P < 0.01) in both two groups. The E-ATP level and E-SOD activity in the PDTC group at 30 and 60 minutes after AC and 30 and 60 minutes after DC were significantly higher than those in control group (P < 0.01). However, the levels of IL-1beta, IL-8, MDA, and F-HB at 30 and 60 minutes after AC and 30 and 60 minutes after DC were significantly lower in the PDTC group than those in control group (P < 0.01). CONCLUSION: PDTC can protect erythrocytes by alleviating lipid peroxidation and inflammatory response during CPB.