| 碱性成纤维细胞生长因子促进ECV304细胞迁移的电镜观察 |
| |
| 引用本文： | 王铭洁,蔡文杰,曹银祥,俞彰,朱依纯. 碱性成纤维细胞生长因子促进ECV304细胞迁移的电镜观察[J]. 复旦学报(医学版), 2005, 32(6): i0011-i0012 |
| |
| 作者姓名： | 王铭洁 蔡文杰 曹银祥 俞彰 朱依纯 |
| |
| 作者单位： | 复旦大学上海医学院生理学与病理生理学系,上海,200032;复旦大学上海医学院电子显微镜室,上海,200032 |
| |
| 基金项目： | 国家“十五”“211工程”重点学科建设基金资助.致谢：感谢电镜室的凌冶萍教授在电镜标本的观察方面给予的许多指导. |
| |
| 摘 要： | 目的 研究碱性成纤维细胞生长因子（bFGF）促进ECV304细胞迁移的作用与细胞形态学变化的关系.方法 建立体外细胞划痕损伤模型,观察bFGF的促进迁移作用.采用扫描电镜和原位包埋后透射电镜分别观察迁移细胞表面和内部细胞器及骨架的变化.结果 bFGF2.5ng/mL促进ECV-304细胞迁移,迁移细胞突起增多,细胞骨架发生重排.结论 bFGF促进ECV-304细胞迁移.
|
| 关 键 词： | 细胞运动 超微结构 成纤维细胞生长因子2 |
| 收稿时间： | 2005-03-08 |
| 修稿时间： | 2005-03-08 |
Observation of the Stimulative Effect of Basic Fibroblast Growth Factor on ECV304 Cell Migration with Electron Microscope |
| |
| WANG Ming-jie,CAI Wen-jie,CAO Yin-xiang,YU Zhang,ZHU Yi-chun. Observation of the Stimulative Effect of Basic Fibroblast Growth Factor on ECV304 Cell Migration with Electron Microscope[J]. Fudan University Journal of Medical Sciences, 2005, 32(6): i0011-i0012 |
| |
| Authors: | WANG Ming-jie CAI Wen-jie CAO Yin-xiang YU Zhang ZHU Yi-chun |
| |
| Abstract: | Purpose To investigate the relationship between morphological change of the ECV304 cells and the promoting effect of the migration process by basic fibroblast growth factor(bFGF). Methods The in vitro cell scratch wound model was set up to observe the promoting effect of the migration process by bFGF.The ultrastructural changes of the cell were observed by scanning and embedding in situ transmission electron microscope. Results bFGF 2.5 ng/mL promoted ECV-304 cell migration.Cell pseudopodia was increased and cell skeleton was rearranged. Conclusions bFGF promoted ECV-304 cell migration. |
| |
| Keywords: | cell movement ultrastructure fibroblast growth factor 2 |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
