阪崎肠杆菌自动化核糖体分型的研究

目的 对阪崎肠杆菌食品分离株进行核糖体分型,并分析其分型效果.方法采用杜邦Riboprinter~(TM)指纹图谱分析仪对2株阪崎肠杆菌标准菌株和28株分离株进行核糖体分型,运用BioNumerics数据库软件建立相应的核糖体指纹图谱数据库,进行指纹图谱分析.结果 核糖体分型将2株阪崎肠杆菌标准菌株和28株分离株分成26种核糖体带型,其中22种带型分别对应1株阪崎肠杆菌试验菌株,其他4种带型分别对应2株试验菌株,不同带型之间的最低相似度为31.58%.所有菌株的酶切条带数为10个左右,酶切条带分子量范围位于1～50 kb之间.同时,使用BioNumerics软件对结果进行分群和多维尺度分析,可见核糖体分型主要分为4个群.结论 自动化核糖体分型可以方便快捷的实现阪崎肠杆菌的分型.

Study on the automatic ribotyping for Enterobacter sakazakii

Objective To analyze the ribotyping fingerprint of Enterobacter sakazakii (E. Sakazakii) isolated from food and its typing power. Methods Two standard strains and twenty-eight isolates of E. Sakazakii were analyzed by the DuPont Riboprinter~(TM) microbial characterization system.The relevant database was established and the fingerprint patterns were analyzed with BioNumerics software. Results This system grouped two standard strains and twenty-eight E. Sakazakii isolates into 26 ribotypes, and four ribotypes included two strains respectively,the other twenty-two strains showed different ribotypes. The lowest similarity was 31.58%. The number of bands by ribotyping was approximately ten and the molecular weight of these bands ranged from 1 to 50 kb. By the clustering program in BioNumerics, these isolates could be grouped into four clusters. Conclusion The automatic ribotyping method is convenient and fast in E. Sakazakii typing.