高碘对桥本甲状腺炎大鼠TRAIL表达的影响

目的:观察高碘环境下桥本甲状腺炎大鼠(EAT)TRAIL的表达在发病机制及病理变化中的作用及意义.方法:采用雌性SD大鼠制作EAT模型,给予高碘饮食,采用免疫组织化学方法和图像分析,检测高碘环境下EAT大鼠甲状腺TRAIL的表达及分布.结果:大鼠甲状腺滤泡细胞中TRAIL的免疫染色阳性率在正常对照组,EAT对照组和EAT高碘组依次增高(P＜0.05).桥本甲状腺炎大鼠中TRAIL免疫染色强阳性甲状腺滤泡细胞多分布于浸润淋巴滤泡附近,浸润淋巴细胞中TRAIL免疫染色较弱.结论:EAT大鼠TRAIL在甲状腺滤泡细胞中呈有特征性分布的高表达,在高碘环境下,更加显著,提示高碘可能通过引起TRAIL表达的增强诱导甲状腺细胞过度凋亡,致甲状腺滤泡破坏.

Effect of iodine excess on TRAIL expression in rats with Hashimoto's thyroiditis

AIM: To explore the significance of the expression of TRAIL in the pathogenesis and pathological changes in the situation of iodine excess in rats with experimental autoimmune thyroiditis(EAT).METHODS: Female SD rats were applied as EAT animal models and fed on high-iodine diets in this study.Immunohistochemical methods were used to detect the expression and distribution of TRAIL in high-level iodine in EAT rats.RESULTS: The positive rates for TRAIL in thyrocytes of rats increased in the order of normal control group,EAT model group and EAT iodine excess group(P<0.05).Strongly positive immunostained thyroid follicular cells of EAT rats for TRAIL mainly distributed adjacently to infiltrating lymphocytes.Staining of infiltrating lymphocytes for TRAIL was found to be rather weak or even absent.CONCLUSION: High characteristic expression of TRAIL in thyroid follicular cells in EAT rats is significant,especially in the presence of iodine excess,which indicates that high-iodine diet might induce thyrocyte apoptosis by increasing the expression of TRAIL,thus resulting in thyroid follicle destruction.