迟发性神经元损伤的实验研究沙土鼠脑海马区组织酶谱及同工酶谱的变化

本文用蒙古沙土鼠制作迟发性神经元损伤(DND)动物模型。在5min脑缺血及缺血后重灌流1～96h,测定了动物脑海马CA_1区组织乳酸脱氢酶(LDH)、α—羟丁酸脱氢酶(α—HBDH)、磷酸肌酸激酶(CK)、谷草转氨酶(GOT)、谷丙转氨酶(GPT)和碱性磷酸酶(ALP)的活性以及LDH及CK同工酶分布的经时变化。本实验的结果进一步证实了能量代谢的变化是DND发生机理中最重要的因素。

EXPERIMENTAL STUDIES ON DELAYED NEURONAL DAMAGE (CHANGES IN ACTIVITIES OF ENZYMES AND ISOENZYMES IN GERBIL HIPPOCAMPUS)

Mongolian gerbils were used as delayed neuronal damage (DND) animal models. At the end of 5-minute cerebral ischemia and at various recirculation times ranging from 1 to 96 hours, activities of LDH, α-HBDH, CK, GOT, GPT and ALP, as well as distributions of LDH and CK isoenzymes, were examined in the CA1 sector of hippocampus. During 3-to 48-hour recirculation, activities of GOT and GPT were significantly increased, but markedly reduced by the end of 95 hours.Relative activities of isoenzymes LDH-5 and CK-MM were increased gradually during the whole recirculation time, and the total activities of LDH, CK and a-HBDH were declined significantly after 48 hours.ALP kept highly active from the beginning to the end of recirculation. These results support further the conclusions we have made in other experiments of ours that change in energy metabolism is the most important factor in the mechanism of DND.