实验性脑缺血及再灌注大鼠脑组织bFGF表达的研究

目的　探讨大鼠脑缺血２ ｈ 后不同时间再灌注脑组织碱性成纤维细胞生长因子（ｂＦＧＦ）的表达程度。方法　采用线栓法栓堵一侧大鼠大脑中动脉，建立脑缺血及再灌注模型。用免疫组化的方法，对缺血脑组织ｂＦＧＦ的表达进行观察。用统计学分析ｂＦＧＦ阳性细胞的数据。结果　正常脑组织中只有微量ｂＦＧＦ的表达；脑缺血２ ｈ 后再灌注０．５ ｈ 起，ｂＦＧＦ少量表达，为神经元表达；脑缺血２ ｈ 后再灌注２２ ｈ，ｂＦＧＦ表达达到高峰，神经元及神经胶质细胞均有表达。脑缺血２ ｈ 后再灌注１６６ ｈ，ｂＦＧＦ仍有持续表达。结论　ｂＦＧＦ参与缺血脑组织的修复过程

The Study of bFGF Expression in Experimental Ischemic/Reperfusion Cerebral Tissue in Rat

Subjective To study the expression of bFGF in ischemic cerebral tissue. Method With the thread occlusion of unilateral middle cerebral artery producing cerebral ischemia and reperfusion, we studied the expression of bFGF in ischemic cerebral tissue with immunohistochemistry techniques.Results The result showed that there were few bFGF expression in nomal cerebral tissue, the expression of bFGF increased after 2 h ischemia and 0 5 h reperfusion, peaked after 2 h ischemia and 22 h reperfusion. The bFGF remained expressed after 2 h ischemia and 166 h reperfusion.Conclusion It is concluded that bFGF may be involved in the process cerebral ischemia.