|
|||||
|
|
| 微囊化NGF基因修饰NIH3T3细胞移植对慢性创面愈合的影响 | |
| 引用本文： | 胡昭华,陈绍宗,金岩,雄鹰,王为,马小军,宋玫. 微囊化NGF基因修饰NIH3T3细胞移植对慢性创面愈合的影响[J]. 组织工程与重建外科, 2005, 1(5): 256-260 |
| 作者姓名： | 胡昭华 陈绍宗 金岩 雄鹰 王为 马小军 宋玫 |
| 作者单位： | 第四军医大学唐都医院整形烧伤科，第四军医大学唐都医院整形烧伤科，第四军医大学口腔医院组织工程实验中心，中国科学院大连化学物理研究所，中国科学院大连化学物理研究所，中国科学院大连化学物理研究所，第四军医大学唐都医院整形烧伤科 |
| 基金项目： | 国家高技术研究发展计划(863计划) |
| 摘 要： | 目的 探讨微囊化转NGF基因NIH3T3细胞移植作用于慢性缺血性创面的方法及其对创面愈合的作用.方法 将转NGF基因的MH3T3细胞包入APA(海藻酸钠-多聚赖氨酸-海藻酸钠)微囊中培养备用,分别采取创面NGF微囊悬液局部注射法、NGF微囊复合组织工程真皮法、MH3T3微囊局部注射法、NGF(5ng/ml)局部注射法、胶原膜法等应用于大鼠背部缺血性创面模型,以空白创面为对照,观察创面再上皮化、愈合速度及愈合时间.结果 两种微囊应用方法均可促进创面上皮化速率,提高创面愈合的速度,与对照组相比差异显著(p＜0.05),其中以微囊复合组织工程真皮法效果最为明显.结论 应用转NGF基因微囊可促进慢性缺血性创面的愈合. |
| 关 键 词： | 微囊 基因转染 神经生长因子 组织工程 慢性缺血性创面 |
| 修稿时间： | 2005-09-08 |
The Effect of Microencapsulated NGF-expressing NIH-3T3 Cells to Promote Chronic Ischemic Wound Healing |
|
| Hu Zhaohua,Chen Shaozong,Song Mei. The Effect of Microencapsulated NGF-expressing NIH-3T3 Cells to Promote Chronic Ischemic Wound Healing[J]. , 2005, 1(5): 256-260 | |
| Authors: | Hu Zhaohua Chen Shaozong Song Mei |
| Affiliation: | Hu Zhaohua,Chen Shaozong,Song Mei. Department of Plastic and Burn Surgery,Tangdu Hospital,Fourth Military Medical University,Xi'an Shanxi,710038,China. Jin Yan. Tissue Engineering Center,Stomatological College,Fourth Military Medical University. Xi'an,Shanxi,710032,China. Xiong Ying,Wang Wei,Ma Xiaojun. Dalian physical and chemical research institute of Academia Sinica,Dalian,Liaoning,116000,China |
| Abstract: | Objective To study the effect of transplantation of the microencapsulated NGF-expressing NIH-3T3 cells to the chronic ischemic wound healing. Methods Using gene transfection technology to obtain NGF-expressing NIH-3T3 cells strain and enclosed in alginate-polylysine-alginate (APA) microcapsule(NGF-MC) in vitro. The model of chronic ischemic circular full thickness excisional wounds on the dorsum of each SD rat was made, and used NGF-MC local injection, NGF-MC incorporated tissue engineering dermis, 3T3-MC local injection, NGF(5ng/ml) local injection, gel membrane dressing respectively to observe the rate of re-epithelization and wound healing and blank wounds were contrast groups. Results NGF-MC can promote the rate of re-epithelization and wound healing compared with other groups regardless of the using method (p<0.05). Furthermore, the method of NGF-MC incorporated with tissue engineering dermis had the best therapeutic effect. Conclusion NGF-expressing NIH3T3 microcapsule can promote the rate of chronic ischemic wound healing. |
| Keywords: | Microencapsulation Gene transfection Nerve growth factor(NGF) Tissue engineering Chronic ischemic wound |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |