Wistar-SD大鼠原位肝移植急性排斥反应的观察

目的 观察Wistar-SD大鼠原位肝移植（OLT）术后发生急性排斥反应时的表现及其判断方法。方法 观察大鼠术后生存状况，采用肝功能检查及组织病理学检查等方法研究OLT模型大鼠发生急性排斥反应的表现，结果Wistar-SD实验组OLT术后发生中，重度急性排斥反应。实验组大鼠肝移植术后血清ALT，AST，TB于第1～3天及第7天各指标数值呈明显上升，高于对照组各相应时点，差异仃统计学意义（P〈0.05）：结论Wistar-SD大鼠OLT模型可发生巾，重度急性排斥反应，其肝功能指标的变化可以说明急性排斥反应是否已发生.

Observations on acute rejection reaction of orthotopic liver transplantation in Wistar-SD rats

Abstract:Objective:To observe the manifestations and judgement methods of acute rejection reaction of orthotopic liver transplantation in Wistar-SD rats.
Methods :The survival conditions after operation in rats was observed, and liver function tests and histopathology examination were used to study the manifestations of acute rejection reaction of orthotopic liver transplantation in Wistar-SD rats.
Results:Wistar-SD experiment group had moderate to severe acute rejection reaction after OLT. Alanine aminotransferase, aspartate aminotransferase, total bilirubin in blood serum after orthotopic liver transplantation in rats was obviously elevated on 1-3 d and 7 d after OLT, which are significantly different from every corresponding time point of control group(P<0.05).
Conclusions:Liver transplantation from donor Wistar rats to recipient SD rats can result in moderate to severe acute rejection reaction, and changes in results of liver functional parameters can indicate whether the acute rejection reaction has occurred.