醋酸棉酚对离体大鼠黄体和人蜕膜、滋养层细胞的影响

以细胞分泌功能和存活率为指标,观察了醋酸棉酚对无血清培养的大鼠黄体和人蜕膜、滋养层细胞的直接损伤作用和可能的作用途径。结果表明:醋酸棉酚对黄体细胞有直接杀伤作用,LD₅₀为:1.6(0.4～2.9)μg·mL^-1。在非致死剂量(0.5μg·mL^-1)下,醋酸棉酚显著抑制黄体细胞基础孕酮分泌,但不能显著抑制hCG,Forskolin的促孕酮作用和3β-HSD的活性。此外,醋酸棉酚对蜕膜细胞和滋养层细胞也有直接杀伤作用,其LD₅₀分别为:3.5(0.4～6.6)μg·mL^-1,4.1(0.6～7.6)μg·mL^-1。本文结果提示,直接杀伤黄体,蜕膜和滋养层细胞并影响孕酮合成是醋酸棉酚抗生育的重要作用环节。

THE EFFECTS OF GOSSYPOL ON RAT LUTEAL CELLS, HUMAN DECIDUAL CELLS AND CYTOTROPHOBLASTS

The direct effects of gossypol on the serum-free primary cultures of rat luteal cells and cytotrophoblasts were observed. The results indicate that: (1) Gossypol affected the viability of the cultured rat luteal cells, LD50: 1.6 (0.4-2.9) micrograms.ml-1. (2) The basal secretions of progesterone were significantly inhibited at low and high concentrations. The activities of 3 beta-HSD, adenyl cyclase and hCG-stimulated progesterone production were inhibited by higher concentrations of gossypol. (3) Gossypol damaged cultured human decidual cells and cytotrophoblasts, the LD50 were 3.5(0.4-6.6) micrograms.ml-1 and 4.1(0.6-7.6) micrograms.ml-1, respectively. These results suggest that the luteolytic effect is the main mechanism of action for the termination of early pregnancy by gossypol, while the direct damaging effects on decidual cells and cytotrophoblasts may also play a role in the termination of early pregnancy.