电针及纳洛酮阻断电针镇痛对豚鼠肾上腺和脊髓脑啡肽样物质影响的免疫组化观察

<正> 自从1975年 Hughes 等发现甲啡肽和亮啡肽以来,许多研究工作已表明,EK 与针刺镇痛关系密切。应用放射免疫(RIA)法的研究表明,大鼠电针后尾核和下丘脑的EK 含量升高,并与针效呈正相关;用抑制 EK 降解的肽酶抑制剂给大鼠腹腔注射(ip)和家兔测脑室注射后,可使针刺镇痛效应提高,同时大鼠尾核和下丘脑 EK 含量升高。Vacca 等曾应用 PAP 法研究了

INFLUENCE OF ELECTROACUPUNCTURE AND ANTAGONISM OF ELECTROACUPUNCTURE ANALGESIA BY NALOXONE ON ENKEPHALIN-LIKE IMMUNOREACTIVITY IN GUINEA PIG'S ADRENAL AND SPINAL CORD

In the present paper,the relationship between the enkephalin-like immuno-reactivity (EK-LIR) and the electroacupuncture (EA) analgesia was studied byimmunohistochemical techniques in guinea pig's adrenal and spinal cord.I.Inaccordance with the pairing design of experiment,56 male guinea pigs weredivided into 28 pairs.Each pair was randomly divided into the test groupwith EA and the control group without EA.2.In accordance with the ran-domized blocks design of experiment,18 male guinea pigs were divided into 6blocks.Each block was randomly divided into 3 groups:1.naloxone plus EAgroup;2.EA group;3.control group without EA.The acupoints were bi-lateral“Zusanli”.The results showed as follows:1.In the test group,the pain threshold after EA increased from 0.26±0.02 mA to 0.47±0.05mA (P<0.001);In the adrenal medulla and spinalcord,both the met-enkephalin (MEK)-LIR and leu-enkephalin (LEK)-LIR inthe test group were much weaker than that in the control group (P<0.01);Correlation analysis between the effect of EA analgesia and the MEK-LIR orLEK-LIR in the adrenal medulla and spinal cord respectively revealed thatthere was a significant negative correlation only between the LEK-LIR andthe effect of EA analgesia (P<0.05).2.After naloxone injection followed by EA,the pain threshold did'tincrease.But it increased obviously after saline injection followed by EA(P<0.01);In the adrenal medulla,the MEK-LIR and LEK-LIR in the naloxoneplus EA group and the EA group were about the same,but much weaker thanthat in the control group withou(?) EA (P<0.01).In the spinal cord,the twokinds of EK-LIR in the naloxone plus EA group and the control group withoutEA were much the same,but much greater than that in the EA group (P<0.01).