miR-206抑制乳腺癌MDA-MB-231细胞Cdc42蛋白表达及其对细胞骨架的影响

目的探讨miR-206对MDA-MB-231乳腺癌细胞Cdc42表达的调控及对细胞骨架的影响。方法采用Li-pofectamineTM2000转染miR-206进入MDA-MB-231细胞,48h后Western blot检测转染后乳腺癌细胞Cdc42、MMP-2和MMP-9蛋白质表达。用免疫荧光显微镜观察细胞丝状伪足的改变,进一步用侵袭迁移实验检测转染前后细胞侵袭力和迁移力的变化。结果Western blot检测Cdc42、MMP-2和MMP-9在转染前后的表达结果,其条带灰度值与阴性对照组相比明显下降(P<0.05);细胞免疫荧光计数每个细胞平均丝状伪足数,空白对照组为(14.99±5.53),表皮生长因子(EGF)组为(23.59±3.92),miR-206转染组为(9.45±3.59),miR-206+EGF组为(11.77±2.85)。与空白对照组和EGF组比较,miR-206转染组或miR-206+EGF组细胞丝状伪足明显减少(P<0.05)。侵袭实验结果显示,小室膜下的细胞数量空白对照组为(311.7±23.5),miR-206转染组为(65.0±13.9),二者差异有统计学意义(P<0.01)...

miR-206 inhibits Cdc42 protein expression, invasion and migration of MDA-MB-231 cells

Objective To investigate whether miR-206 can regulate Cdc42 protein expression and cytoskeleton remodeling. Methods miR-206 was transfected into MDA-MB-231 cells using LipofectamineTM 2000, and the transfected cells were harvested in 48 h. Western blot analysis was used to detect the protein expressions of Cdc42, MMP-2 and MMP-9. Actin-Tracker Green was used to stain F-actin and filopodia was observed by fluorescent microscopy. Invasion assay and Transwell migration assay were taken to examine the invasive ...