双酚A对大鼠背根神经节神经元钙离子通道电流影响 |
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引用本文: | 王文娟,王军,钱文溢,朱勤,肖杭.双酚A对大鼠背根神经节神经元钙离子通道电流影响[J].中国公共卫生,2012,28(2):210-212. |
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作者姓名: | 王文娟 王军 钱文溢 朱勤 肖杭 |
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作者单位: | 南京医科大学公共卫生学院现代毒理教育部重点实验室,江苏南京,210009 |
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基金项目: | 国家自然科学基金,江苏省普通高校研究生科研创新计划 |
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摘 要: | 目的探讨双酚A对大鼠背根神经节(DRG)神经元钙离子通道影响。方法 SD大鼠,周龄4~6周,采用酶消化法急性分离大鼠背根神经节神经元,分别采用全细胞膜片钳技术和激光共聚焦技术记录钙电流和KCl激发钙瞬变的变化。结果双酚A(0.1、1、10、100μmol/L)呈浓度依赖性抑制大鼠背根神经节神经元钙电流,对钙通道的半数最大抑制浓度(IC50)为11.41μmol/L;10μmol/L双酚A显著改变Ca2+通道的激活特性,电流激活曲线去极化方向移动(P<0.05),该浓度双酚A同样可以抑制50 mmol/L KCL激发瞬时胞内钙浓度增加。结论双酚A对大鼠背根神经节神经元钙离子通道具有抑制作用。
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关 键 词: | 双酚A(BPA) 钙通道 膜片钳 激光共聚焦 钙瞬变 |
Effects of bisphenol A on calcium channel currents in dorsal root ganglion neurons of rats |
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Institution: | WANG Wen-juan,WANG Jun,QIAN Wen-yi,et al.The Ministry of Education Key Lab of Modern Toxicology,School of Public Health,Nanjing Medical University(Nangjing 210029,China) |
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Abstract: | Objective To explore the effects of bisphenol A(BPA) on calcium currents in dorsal root ganglion(DRG) neuron cells of rats.Methods Ca2+ currents were obtained in DRG neurons by whole-cell patch clamp technique;calcium transients induced by 50 mmol/L KCl were also observed in the neurons with laser scanning confocal microscopy assay,and the effects of bisphenol A on calcium channels were observed.Results BPA at the concentrations of 0.1,1,10,and 100 μmol/L significantly inhibited calcium current in DRG neurons cells,with an IC50 of 11.41 μmol/L.BPA shifted the activation curve of calcium currents toward a depolarizing direction and increased the slope factor of the curve.Additionally,10μmol/L BPA inhibited the increment of intracellular Ca2+ concentration(i) induced by 50 mmol/L KCl.Conclusion BPA has significant inhibitory effects on calcium current and calcium transient invoked by KCl in dorsal root ganglion neurons. |
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Keywords: | bisphemol A calcium channel patch clamp laser scanning confocal microscopy calcium transient |
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