辽宁省水痘-带状疱疹病毒基因型分析

目的了解辽宁省水痘-带状疱疹病毒(VZV)基因型别。方法选取水痘患者临床确诊病例7例,采集血清和咽拭子标本,分别检测VZV IgM抗体、扩增ORF22基因VZV核酸片断,进行核苷酸序列测定分析,测序结果与GenBank的VZV参考株基因序列进行比较。结果 7例临床确诊病例VZV IgM抗体均阳性(编号为VZV001～VZV007);其中3例的咽拭子标本扩增到目的片段(VZV001、VZV-002、VZV-005),测序结果证实为水痘-带状疱疹病毒;ORF22基因的单核苷酸多态性(SNP)分析显示,VZV-002与VZV-005基因序列完全一致,且在同一位点的碱基由C代替了A;VZV-001序列2个位点出现碱基代替,提示其基因可能发生点突变;与Dumas株序列比较,3株分离株在6个关键位点上有4个核苷酸不同,与P-Oka株则完全一致,3株VZV分离株的ORF22基因均鉴定为J基因型,与水痘-带状疱疹病毒P-Oka株的同源性为99.56%～99.78%。结论辽宁省检测到的3株VZV基因型均为J型。

Genotype analysis of varicella-zoster virus in Liaoning province

Objective To examine the genetype of varicella-zoster virus in Liaoning province.Methods The serum and throat swabs speciments were collected from 7 patients clinically diagnosed with varicella for the detection varricella-zoster virus(VZV) IgM and the amplification of DNA of the open reading frame(ORF)22.The DNA was sequenced and analyzed with the data of VZV reference strains downloaded from GenBank.Results All the 7 patients were VZV IgM positive and the amplification,and sequencing of 3 throat swabs(VZV001,VZV-002,VZV-005) confirmed the VZV.The results of ORF22 single nucletotide polymorphism(SNP) analyses showed that VZV-002 and VZV-005 had the same sequence and the single base transvertion was A→C.The sequence of VZV-001 had 2 changed bases,suggesting the point mutation of the gene.Compared with Dumas strain,the 3 strains had 4 bases different on 6 key site and completely matched with P-Oka strain.The 3 strains were identified as J genotype and the nucleotide homology was 99.56%-99.78% with P-Oka.Conclusion The genotype of 3 VZV strains detected in Liaoning province belongs to J genetype.