尖锐湿疣患者外周血CD8+T细胞细胞因子的表达

目的 检测尖锐湿疣患者外周血CD8⁺T细胞白介素-2、白介素-12、干扰素-γ、白介素-4的表达水平,探讨其在尖锐湿疣发病中的作用.方法 应用流式细胞仪检测60例尖锐湿疣患者和20例正常人对照者外周血CD8⁺T细胞白介素-2、白介素-12、干扰素-γ、白介素-4的表达水平.结果 尖锐湿疣患者外周血CD8⁺T细胞白介素-2、白介素-12、干扰素-γ阳性细胞百分比均显著下降(P<0.01);白介素-4+CD8⁺T细胞百分比与正常人对照组比较,差异无统计学意义(P>0.05);干扰素-γ+/白介素-4+T细胞比值显著低于正常人对照组(P<0.01).结论 尖锐湿疣患者Tc1细胞水平低下,Tc1/Tc2显著低于正常人对照.

Expression of cytokines in CD8+ T lymphocytes of the peripheral blood of patients with condyloma acuminatum

Objective To detect the levels of cytokine IL-2, IL-12, IFN-γ and IL-4 secreted by peripheral CD8+ T lymphocytes in patients with condyloma acuminatum （CA）. Methods Flow cytometry was employed to study the expression of cytokines IL-2, -12, INF-γ and IL-4 in CD8+ T lymphocytes in the peripheral blood of 60 patients with CA and 20 healthy controls. Results The percentage of CD8+ T lymphocytes producing IL-2, IL-12 and IFN-γ were significantly lower in CA patients than that in healthy controls （P < 0.001）, while the percentage of IL-4-producing CD8+ T lymphocytes was of no significant difference between two groups. The ratio of Tc1/Tc2 was also decreased in CA patients as compared with the healthy controls （P < 0.001）. Conclusion The quantity of Tc1 cells and the ratio of Tc1/Tc2 are significantly lower in CA patients than those in healthy controls.