Kaposi肉瘤组织内人类疱疹病毒8型的K15基因型研究

目的 了解Kaposi肉瘤组织中人类疱疹病毒8型(HHV-8)K15等位基因型分布情况,并初步探讨Kaposi肉瘤不同临床分型及临床表现与不同HHV-8 K15等位基因型的相关性.方法 采用酚-氯仿-异戊醇法对收集的27例Kaposi肉瘤石蜡包埋组织标本进行病毒DNA抽提,并使用巢式PCR扩增K15基因片段,然后测序并确定其等位基因型.结果 27例Kaposi肉瘤中有22例HHV-8感染为阳性,阳性率为81.48%,其中4例艾滋病-Kaposi肉瘤患者HHV-8感染均为阳性,感染率100%;在分析的22例HHV-8病毒株中,20例为P型,2例为M型;4例艾滋病-Kaposi肉瘤患者感染的均为P型HHV-8,2例M型感染者均为经典型Kaposi肉瘤患者.结论 Kaposi肉瘤组织内HHV-8的K15等位基因型主要是P型,也存在部分M型HHV-8感染者.4例艾滋病-Kaposi肉瘤患者感染的HHV-8均为P型.

Genotyping of K15 gene of human herpesvirus 8 in Kaposi's sarcoma

Objective To study the distribution of K15 alleles of human herpesvirus 8 （HHV-8） in Kaposi′s sarcoma （KS） in Xinjiang, and to investigate the relationship between clinical profiles of KS and alleles of HHV-8 K15. Methods HHV-8 DNA was extracted with phenol-chloroform-isoamyl alcohol from 27 formalin-fixed, paraffin-embedded tissue specimens of KS. The HHV-8 K15 gene was amplified by nested- PCR, and sequenced for the identification of K15 allele. Results HHV-8 DNA could be detected in 22 （81.48%） out of 27 KS patients in Xinjiang. HHV-8 DNA was detected in all 4 patients with AIDS-related KS. Twenty viral strains were identified as P type, including all 4 from the AIDS-related KS patients; two strains were identified as M type, which were all from the classical KS patients. Conclusions In KS, most of HHV-8 K15 alleles are P type, and some are M type. The 4 patients with AIDS-related KS all carried P type of K15 allele.