女性生殖道尖锐湿疣患者外周血淋巴细胞B7-CD28/CTLA-4的表达及意义

目的 探讨B7-CD28/CTLA-4(CD152)T淋巴细胞活化协同刺激分子在女性生殖道尖锐湿疣发病、发展及转归中的作用.方法 用流式细胞仪检测30例女性生殖道尖锐湿疣患者和15例正常人外周血淋巴细胞中CD80/CD86及CD4⁺/CD8⁺T淋巴细胞中CD28/CD152(CTLA-4)的表达.结果 CD80/CD86及CD4⁺、CD8⁺/CD28阳性表达水平初发组(17例)、复发组(13例)与正常人对照组(15例)比较,差异无统计学意义;恢复组(15例)CD4⁺、CD8⁺/CD28及CD80阳性表达水平分别较复发组明显增高(P<0.05).CD4⁺、CD8⁺/CD152在尖锐湿疣复发组和初发组阳性表达水平均较正常人对照组明显增高(P<0.05),且复发组较初发组进一步增高,两组比较差异有统计学意义(P<0.05);恢复组CD4⁺、CD8⁺/CD152阳性表达水平较初发组及复发组明显降低(P<0.05),与正常人对照组比较差异无统计学意义.结论 女性生殖道尖锐湿疣患者外周血淋巴细胞和CD4⁺、CD8⁺T淋巴细胞中B7-CD28/CTLA-4(CD152)表达异常,并与其病程发展关系密切.

Expression of B7-CD28/CTLA-4 in peripheral blood lymphocytes of female patients with genital condyloma acuminatum

Objective To investigate the significance of B7-CD28/CTLA-4 （CD152）, co-stimulatory molecules of T lymphocytes, in the onset, development and prognosis of genital congdyloma acuminatum （CA） in females. Methods Flow cytometry was utilized to detect the expression levels of CD80/CD86 in peripheral blood lymphocytes and of CD28/ CD152（CTLA-4） in CD4+/CD8+ T lymphocytes from 30 CA patients （17 primary CA, 13 recurrent CA, 15 at recovery stage of CA） and 15 healthy volunteers as controls. Results No significant difference was found for the frequencies of CD80+, CD86+, CD4+/CD28+ and CD8+/CD28+ lymphocytes between the primary or recurrent group and the control group. The frequencies of CD8+/CD28+, CD4+/CD28+ and CD80+ lymphocytes were significantly higher in the recovery group than those in the recurrent group （P < 0.05）. The frequencies of CD4+/CD152+ and CD8+/CD152+ cells were significantly lower in the primary group than those in the recurrent group ［（10.01 ± 1.62）% vs （14.85 ± 2.38）%, （9.09 ± 1.20）% vs （14.91 ± 1.87）%, both P < 0.05］, but significantly higher than those in the recovery ［（10.01 ± 1.62）% vs （5.30 ± 0.43）%, （9.09 ± 1.20）% vs （4.95 ± 0.63）%, both P < 0.05］ and control groups ［（10.01 ± 1.62）% vs （1.75 ± 0.10）%, （9.09 ± 1.20）% vs （3.92 ± 0.53）%, both P < 0.05］, and no significant difference of those cells was found between the control and recovery group （P > 0.05）. Conclusions There is an abnormal expression of co-stimulatory molecules B7-CD28/ CTLA-4 （CD152） in periphoral blood lymphocytes, CD4+ and CD8+ T lymphocytes in female patients with genital CA, and the expression abnormaility is closely linked with different disease stages of CA.