| 参附注射液对人慢性髓性白血病细胞来源的树突状细胞诱导生成的影响 |
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| 引用本文： | 任莉莉,问明,杨华,臧爱民,贾友超,魏影非.参附注射液对人慢性髓性白血病细胞来源的树突状细胞诱导生成的影响[J].山东医药,2011,51(52):34-36. |
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| 作者姓名： | 任莉莉 问明 杨华 臧爱民 贾友超 魏影非 |
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| 作者单位： | 1. 河北大学附属医院,河北保定,071000
2. 河北医科大学第三医院 |
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| 摘 要： | 目的研究参附注射液（SFI）对慢性髓性白血病细胞系K-562细胞诱导生成树突状细胞（DCs）的影响。方法用不同浓度的SFI、粒—巨噬细胞集落因子（GM-CSF）/白细胞介素4（IL-4）及GM-CSF/IL-4联合不同浓度SFI 3种组合在体外诱导培养K-562细胞。结果体外单用SFI不能诱导K-562细胞分化为DCs。单用GM-CSF/IL-4可诱导K-562细胞分化为DCs,并具有DCs的特异性标志及功能,但不成熟。不同浓度的SFI联合GM-CSF/IL-4对K-562 DCs有促进成熟和抑制增殖的双向调节作用,其最佳诱导浓度为75 mg/ml。结论适当浓度的SFI联合GM-CSF/IL-4在体外能更有效地诱导K-562细胞分化为DCs,并促进DCs成熟,增强DCs功能,且75 mg/mlSFI与GM-CSF/IL-4联合有协同作用。
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| 关 键 词： | 参附注射液 白血病 树突状细胞 K-562细胞 |
Promotion of shenfu Injection on inducing chronic myeloid leukemia cell into dendritic cells |
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| REN Li-li
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WEN Ming
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YANG Hua
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ZANG Ai-min
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JIA You-chao
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WEI Ying-fei.Promotion of shenfu Injection on inducing chronic myeloid leukemia cell into dendritic cells[J].Shandong Medical Journal,2011,51(52):34-36. |
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| Authors: | REN Li-li WEN Ming YANG Hua ZANG Ai-min JIA You-chao WEI Ying-fei |
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| Institution: | REN Li-li1,WEN Ming,YANG Hua,ZANG Ai-min,JIA You-chao,WEI Ying-fei(1 Affiliated Hospital of Hebei University,Baoding 071000,P.R.China) |
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| Abstract: | Objective To investigate the effects of shenfu injection(SFI) on the induction of chronic myeloid leukemia cell line K-562 cells into dendritic cells(DCs).Method In vitro,K-562 cells were cultured with different concentrations SFI,granulocyte-macrophage colony-stimulating factor(GM-CSF)/interleukin-4(IL-4) and GM-CSF/IL-4 combined with different concentrations of SFI,respectively.Results In vitro,DCs could not be derived from K-562 cells cultured with different concentrations of SFI.While DCs derived from K... |
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| Keywords: | shenfu injection leukemia dendritic cells K-562 cell |
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