| 以Cathelicidin序列为模板设计合成抗菌肽的抗菌特性研究 |
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| 引用本文： | 陈曼,胡启飞,陈登榜,吴民泸,代娟,赖翼,段佳慧.以Cathelicidin序列为模板设计合成抗菌肽的抗菌特性研究[J].山东医药,2011,51(52):19-21. |
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| 作者姓名： | 陈曼 胡启飞 陈登榜 吴民泸 代娟 赖翼 段佳慧 |
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| 作者单位： | 1. 成都医学院,成都,610083
2. 丽珠集团四川光大制药有限公司 |
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| 基金项目： | 四川省教育厅科研项目(09ZC078) |
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| 摘 要： | 目的研究以Cathelicidin序列为模板设计合成的抗菌肽对金黄色葡萄球菌的抗菌特性。方法根据NCCLS的方法,测定合成的抗菌肽对游离金黄色葡萄球菌的最低抑菌浓度（MIC）和最低杀菌浓度（MBC）;配制5%的红细胞悬液,与抗菌肽混合孵育后测定540 nm的吸光度;金黄色葡萄球菌的过夜培养物加入80μg/ml的抗菌肽,37℃培养4 h,每隔1 h测定菌落数,比较在抗菌肽作用下活菌数随时间的变化。结果抗菌肽的MIC为16μg/ml,MBC为128μg/ml;100μg/ml抗菌肽的溶血性小于10%;抗菌肽作用4 h后,金黄色葡萄球菌活菌数（5.63±0.10）lg（cfu/ml）,与对照组相比有显著性差异（P〈0.05）。结论通过序列设计人工合成的抗菌肽对金黄色葡萄球菌有较强的活性,且溶血性较小。
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| 关 键 词： | 抗菌肽 金黄色葡萄球菌 抑菌作用 |
Biological activities of a synthetic antimicrobial peptide derived from the cathelicidin family |
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| CHEN Man
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HU Qi-fei
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CHEN Deng-bang
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WU Min-lu
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DAI Juan
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LAI Yi
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DUAN Jia-hui.Biological activities of a synthetic antimicrobial peptide derived from the cathelicidin family[J].Shandong Medical Journal,2011,51(52):19-21. |
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| Authors: | CHEN Man HU Qi-fei CHEN Deng-bang WU Min-lu DAI Juan LAI Yi DUAN Jia-hui |
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| Institution: | CHEN Man1,HU Qi-fei,CHEN Deng-bang,WU Min-lu,DAI Juan,LAI Yi,DUAN Jia-hui(1 Chengdu College of Medicine,Chengdu 610083,P.R.China) |
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| Abstract: | Objective To study the effect of synthetic antimianrobial peptide derived from the Cathelicidin family on staphyoccocus aureus.Methods According to the method of NCCLS,the minimal inhibitory concentration(MIC) and the minimal bactericidal concentration(MBC) of the synthetic antimicrobial peptide against staphyoccocus aureus were determined.Peptide was incubated with a freshly prepared erythrocyte suspension from human blood(5% of normal hematocrit) and released hemoglobin which was determined photometricall... |
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| Keywords: | antimicrobial peptide staphyloccocus aureus antibacterial activity |
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