| 杜仲对单侧输尿管梗阻大鼠肾间质纤维化的影响 |
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| 引用本文： | 丁庆余,宋恩峰,贾汝汉.杜仲对单侧输尿管梗阻大鼠肾间质纤维化的影响[J].中国中西医结合肾病杂志,2010,11(2):109-111,I0003. |
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| 作者姓名： | 丁庆余 宋恩峰 贾汝汉 |
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| 作者单位： | 1. 武汉大学人民医院中西医结合科,武汉,430060
2. 武汉大学人民医院肾内科,武汉,430060 |
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| 摘 要： | 目的：观察杜仲对单侧输尿管梗阻（unilateral ureteral obstruction,UUO）大鼠肾间质纤维化的影响并探讨其可能机制。方法：采用单侧输尿管梗阻（UUO）肾间质纤维化模型,将32只大鼠随机分为4组,每组8只,即假手术组、模型组、替米沙坦组和杜仲组。替米沙坦组给予替米沙坦10 mg.kg-1.d-1、杜仲组给予杜仲6 g.kg-1.d-1治疗。术后2周处死大鼠,收集血清测定尿素氮（BUN）、血肌酐（Scr）,光镜下观察Masson和HE染色肾间质的病理改变,并应用免疫组织化学方法检测肾组织α-平滑肌肌动蛋白（α-SMA）和纤维连接蛋白（FN）的表达。结果：模型组与两治疗组大鼠血清Scr、BUN上升,与假手术组比较差异有统计学意义（P〈0.05）;模型组、杜仲组、替米沙坦组肾间质纤维化程度均较假手术组明显增高（P〈0.05）;模型组α-SMA、FN的表达增加,与假手术组相比差异有统计学意义（P〈0.01）;杜仲组与模型组相比,α-SMA、FN的表达下降,差异有统计学意义（P〈0.05）;杜仲组与替米沙坦组各指标比较差异均无统计学意义（P〉0.05）。结论：杜仲能降低UUO大鼠BUN、Scr水平,改善肾功能;能够抑制α-SMA和FN在UUO大鼠肾组织中表达上调,减少UUO大鼠肾间质纤维化面积。
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| 关 键 词： | 杜仲 肾间质纤维化 单侧输尿管梗阻 α-平滑肌肌动蛋白 纤维连接蛋白 |
Effect of Eucommia on Expression of Renal Interstitial Fibrosis in Rats with Unilateral Urethral Obstruction |
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| DING Qingyu,SONG Enfeng,JIA Ruhan.Effect of Eucommia on Expression of Renal Interstitial Fibrosis in Rats with Unilateral Urethral Obstruction[J].Chinese Journal of Integrated Traditional and Western Nephrology,2010,11(2):109-111,I0003. |
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| Authors: | DING Qingyu SONG Enfeng JIA Ruhan |
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| Abstract: | Objective:To investigate the effects of Eucommia on renal interstitial fibrosis and its mechanism.Methods:Models of renal interstitial fibrosis in rats were established with unilateral urethral obstruction(UUO).Adult male SD rats(n=32) were randomly divided into four groups:the sham operation group(Sham),the UUO group,the Telmisartan group,and the Eucommia group.The rats were sacrificed 2 weeks after surgery.The rats' blood were sampled for determination of BUN and Scr levels,and the renal tisssue of the ra... |
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| Keywords: | Eucommia Renal interstitial fibrosis Unilateral urethral obstruction α-SMA FN |
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