细菌对模拟胆汁热力学平衡的影响

目的:探讨细菌对模拟胆汁物理鄄化学平衡的影响。方法:观察加入大肠杆菌、粪肠球菌、铜绿假单胞菌和生理盐水的模拟胆汁中胆固醇晶体形成的时间和胆汁成分析出黏附的时间,测量胆汁黏度值和胆汁类脂成分溶解度的变化。结果:加入粪链球菌或铜绿假单胞菌的模拟胆汁,黏度值增加,试管壁上有黏附物质析出。加入大肠杆菌或生理盐水的模拟胆汁中,无类似变化。在不同的胆固醇饱和条件下,黏附物质析出的时间早于或等于胆固醇晶体形成的时间。胆固醇溶解度在加入粪链球菌或铜绿假单胞菌的模拟胆汁中,低于在加入大肠杆菌或生理盐水的模拟胆汁;胆汁酸盐或磷脂的溶解度无显著差异。胆汁析出的黏附物质中胆固醇是主要成分之一。结论:粪链球菌或铜绿假单胞菌增加模拟胆汁的黏度并有黏附物质形成,而降低胆固醇的溶解度。

The influence of addition of bacteria on the morphologic change and the thermodynamic balance of the model bile

Objective To investigate the influence of addition of bacteria on the morphologic change and the physical-chemistry balance of the model bile. Methods Observation of the morphologic change of the bile and onset time of cholesterol crystal formation and appearance of adhesion in the model bile when E.coli, Enterococcus faecalis, Pseudomonas aeruginosa or saline were added. Determination of the change of bile viscosity and solubility of bile lipids. Results The viscosity of the model bile increased with appearance of adhesive material on tube wall when Enterococcus faecalis or Pseudomonas aeruginosa was added; while no such phenomenon was observed in the model bile when E.coli or saline was added. The onset time of adhesion was shorter than or equal to the onset time of cholesterol crystal formation under different cholesterol saturation conditions. The solubility of cholesterol in the model bile when added with Enterococcus faecalis or Pseudomonas aeruginosa was lower than that of the bile when added with E.coli or saline, while no difference of solubility of bile acid or lecithin was oberserved between the 2 groups. Cholesterol was the main component of the adhesive material. Conclusions Addition of Enterococcus faecalis or Pseudomonas aeruginosa will enhance the adhesiveness of the model bile and the formation of adhesive material, while lowering the solubility of cholesterol.