胸腺修饰诱导同种异体静脉移植的免疫耐受研究 |
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引用本文: | 孟庆刚,孙丕云,岳伟杰,胡成乙,王立群. 胸腺修饰诱导同种异体静脉移植的免疫耐受研究[J]. 中华实验外科杂志, 2008, 26(1): 895-897. DOI: 10.3760/cma.j.issn.1001-9030.2009.07.028 |
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作者姓名: | 孟庆刚 孙丕云 岳伟杰 胡成乙 王立群 |
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作者单位: | 哈尔滨医科大学附属第四医院骨外科,150001;哈尔滨医科大学附属第四医院病理科,150001;哈尔滨医科大学基础医学院免疫教研室; |
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基金项目: | 黑龙江省自然科学基金资助项目 |
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摘 要: | Objective To investigate the possibility of inducing immune tolerance to vein trans-plantation in rats by intrathymic injection of allogene. Methods MHC antigen extracted from splenic cells of donor Wistar rats was intrathymically injected to recipients SD rats, and donor Wistar femur vein was transplanted after 2 weeks. Forty-eight SD rats were randomly divided into 4 groups: group A ( femur vein autograft) ,group B ( femur vein allograft) ,group C ( femur vein allograft with use of immunosuppressant), group D (intrathymus injection). Imaging, histology and immunological assays of these groups were carried out 2 weeks after the transplantation. Results Histologic parameters that were tested were better in group D than those in group B. The concentration of serum IFN-γ in group B was significantly higher than that in groups A, C and D [ ( 86. 707±10.928 ) ng/L vs (29.328±4. 170), (69.076±8.059) and (63.355± 4.895) ng/L respectively, P < 0.05 ]. The concentration of serum IL-4 in group B was obviously lower than in groups C and D [ (23.656±3.369) ng/L vs (29.425±4.174) and ( 31.000±4.659) ng/L re-spectively,P < 0.05 ]. Conclusion Intrathymus injection of allogenic antigen might induce specific im-mune tolerance to femur vein transplantation in rats.
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关 键 词: | 胸腺 抗原 静脉移植 免疫耐受 |
Inducing transplantation immune tolerance to rats vein by intrathymic injection of allogenic antigen |
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Abstract: | |
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Keywords: | ThymusAntigenVein transplantationImmunological endurance |
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