| 基于超高效液相色谱-飞行时间质谱的CCl_4诱导肝损伤小鼠血浆代谢组学研究 |
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| 引用本文： | 陈汀,姚卫峰,张丽,丁安伟.基于超高效液相色谱-飞行时间质谱的CCl_4诱导肝损伤小鼠血浆代谢组学研究[J].中国实验方剂学杂志,2010,16(18):98-101. |
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| 作者姓名： | 陈汀 姚卫峰 张丽 丁安伟 |
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| 作者单位： | 1. 南京中医药大学药学院,南京,210046
2. 南京中医药大学药学院,南京,210046;江苏省方剂研究重点实验室,南京,210046 |
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| 基金项目： | 国家自然科学基金资助项目(81001599)；科技部重大新药创制(2009ZX09103-339); 江苏省中医药管理局研究项目(LB09031) ; 南京中医药大学青年自然科学基金项目(09XZR14) |
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| 摘 要： | 目的:利用代谢组学的方法研究不同剂量CCl4诱导的小鼠肝损伤模型,确定能够表征CCl4肝损伤模型的血浆代谢物中的生物标记物。方法:采用基于超高效液相色谱-飞行时间质谱(UPLC-TOF-MS)的代谢组学技术,并结合主成分分析的方法,研究正常小鼠和不同剂量CCl4诱导的肝损伤小鼠血浆代谢物组的变化。结果:初步确定建立小鼠肝损伤血浆代谢物研究模型的CCl4用量,并寻找到10个具有特征性的内源性生物标记物。结论:代谢组学方法成功用于CCl4诱导的小鼠血浆肝损伤模型研究,并为进一步研究中药的保护肝脏作用奠定了基础。
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| 关 键 词： | 代谢物组学 UPLC-TOF-MS 肝损伤 小鼠血浆 生物标记物 |
| 收稿时间： | 2010/8/31 0:00:00 |
UPLC-TOF-MS Based Metabonomic Study of CCl4-induced Rat-plasma Liver Injury |
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| CHEN Ting,YAO Wei-feng,ZHANG Li and DING An-wei.UPLC-TOF-MS Based Metabonomic Study of CCl4-induced Rat-plasma Liver Injury[J].China Journal of Experimental Traditional Medical Formulae,2010,16(18):98-101. |
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| Authors: | CHEN Ting YAO Wei-feng ZHANG Li and DING An-wei |
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| Abstract: | Objective: Use the metabonomics method to study the CCl4-induced liver injury model, and discriminate the potential biomarkers contributed to liver injury in plasma metabolites. Method: Adopt the metabolic skills based on UPLC-TOF-MS and PCA, to research the change of plasma metabolite in normal rat or CCl4-induced liver injury rat with two different doses. Result: Ascertain the CCl4 dosage in establish rat liver injury model of plasma metabolites and find ten characteristic biomakers in plasma. Conclusion: Metabonomics method was successfully used to study the CCl4-induced liver injury model by the rat plasma, and laid a foundation for the further study of the liver protection of Traditional Chinese Medicine. |
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| Keywords: | metabonomics UPLC-TOF-MS liver injury rat plasma biomarker |
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