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Propolis induces cell cycle arrest and apoptosis in human leukemic U937 cells through Bcl-2/Bax regulation |
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| Authors: | Motomura Muneo Kwon Kyung Min Suh Seok-Jong Lee Young-Choon Kim Yeon-Kye Lee In-Seon Kim Myung-Sunny Kwon Dae Young Suzuki Ikukatsu Kim Cheorl-Ho |
| Affiliation: | aDepartment of Clinical Nutrition, Suzuka University of Medical Science, 1001-1 Kishioka, Suzuka City, Mie 510-0293, Japan bDepartment of Biological Sciences, Sungkyunkwan University, 300 Chunchun-dong, Suwon, Kyunggi-Do 440-746, Republic of Korea cDepartment of Biotechnology, Dong-A University, Hadan-dong, Saha-Gu, Busan 608-714, Republic of Korea dLaboratory of Bioactive Resources, Biotechnology Research Institute, National Fisheries Research and Development Institute, 408 Sirangri, Gijang, Haeundae, Busan 619, Republic of Korea eThe Center for Traditional Microorganism Resources, Keimyung University, Daegu 704-701, Republic of Korea fKorea Food Research Institute, Songnam, Kyongki-Do 463-420, Republic of Korea |
| Abstract: | We investigated mechanism(s) where propolis induces apoptosis in human leukemic U937 cells. Propolis inhibited the proliferation of U937 cells in a dose-dependent manner by inducing apoptosis and blocking cell cycle progression in the G2/M phase. Western blot analysis showed that propolis increases the expression of p21 and p27 proteins, and decreases the levels of cyclin B1, cyclin A, Cdk2 and Cdc2, thereby contributing to cell cycle arrest. DAPI staining assay revealed typical morphology features of apoptotic cells. Propolis-induced apoptosis was also confirmed by assays with annexin V-FITC, PI-labeling and DNA fragmentation assay. The increase in apoptosis level induced by propolis was associated with down-regulation of Bcl-2 and activation of caspase-3, but not with Bax. These results suggests that propolis-induced apoptosis is related to the selective activation of caspase-3 and induction of Bcl-2/Bax regulation. |
| Keywords: | Propolis Bcl-2/Bax Caspase-3 Human leukemic U937 Apoptosis |
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