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四倍体白芷药材中欧前胡素的含量测定
引用本文:彭菲,张胜,刘塔斯,肖冰梅.四倍体白芷药材中欧前胡素的含量测定[J].中国中药杂志,2002,27(6):426-427.
作者姓名:彭菲  张胜  刘塔斯  肖冰梅
作者单位:湖南中医学院,药学院,湖南,长沙,410004
基金项目:湖南省科技厅资助项目 (97SSY10 0 5 )
摘    要:目的 :测定四倍体白芷药材中欧前胡素的含量 ,并与原二倍体白芷相比较。方法 :采用高效液相色谱法 ,色谱柱Nova pak 4.6mm×20 0mm ,5μm ,流动相乙腈 水 ,流速 1mL·min-1,检测波长 248nm ,柱温 25℃。结果 :四倍体白芷药材中欧前胡素含量达 0.46% ,二倍体对照药材仅为 0.225% ,含量增加了 104.4%。结论 :人工四倍体白芷主要有效成分含量成倍提高。

关 键 词:四倍体白芷  欧前胡素  高效液相色谱
文章编号:1001-5302(2002)03-0426-02
收稿时间:2001/11/12 0:00:00

Determination of Imperatorin in Tetraploidy Radix Angelicae dahuricae
PENG Fei;ZHANG Sheng;LIU Ta si;XIAO Bing mei.Determination of Imperatorin in Tetraploidy Radix Angelicae dahuricae[J].China Journal of Chinese Materia Medica,2002,27(6):426-427.
Authors:PENG Fei;ZHANG Sheng;LIU Ta si;XIAO Bing mei
Institution:Hunan College of TCM, Changsha 410004, Hunan, China.
Abstract:OBJECTIVE: To determine the content of imperatorin in the tetraploidy radix angelicae dahuricae, and compare it with the original diplontic varites. METHOD: The chromatographic method was carried out on Nova-pak (4.6 mm x 200 mm, 5 microns) column with acetonitrile-water solution as a mobile phase. The flow rate was 1 ml.min-1. the detection wavelength was at 248 nm, and the column temperature was 25 degrees C. RESULT: The eontent of was imperatorin in the tetraploidy radix 0.460% and 0.225% imperatorin in the diplontic species. CONCLUSION: The content of the mainly active constituent in the tetraploid is double to what it is in the original diplontic species.
Keywords:tetraploidy radix angelicae dahuricae  imperatorin  HPLC
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