解毒祛瘀滋阴药对MRL／lpr小鼠外周血淋巴细胞凋亡及线粒体跨膜电位的影响

目的探讨解毒祛瘀滋阴中药对MRL／lpr小鼠外周血淋巴细胞凋亡及其线粒体跨膜电位的影响。方法80只MRL／lpr小鼠随机分为：模型组：20只，灌胃0．9％氯化钠注射液；中药组：20只，灌胃解毒祛瘀滋肾中药煎剂；西药组：20只，灌胃强的松混悬液；中西药组：20只，中西药分别灌胃；正常组：昆明小鼠选用20只，0．9％氯化钠注射液灌胃，均每日1次，每次0．5ml。连续饲养用药12周后取血，以梯度离心法分离提纯外周血淋巴细胞（PBLC），培养48h，流式细胞仪检测凋亡率及其线粒体跨膜电位水平。结果正常组、中药组、西药组和中西药组PBLC0和48h凋亡率高于模型组，差异有显著性（P〈0．05，P〈0．01）。0小时西药组、正常组与中西药组的凋亡率无明显差异（P〉0．05），但中药组和模型组与中西药组比较，差异有显著性（P〈0．05）；48h西药组与中西药组的凋亡率有明显差异（P〈0．05），中药组和模型组与中西药组比较，差异有显著性（P〈0．01）；正常组、中药组、西药组和中西药组PBLC线粒体跨膜电位水平水平均明显低于模型组，差异有显著性（P〈0．05和P〈0．01）；西药组与中西药组低于正常组，模型组高于正常组，且比较均差异有显著性（P〈0．05和P〈0．01）；中药组与正常组之间差异无显著性（P〉0．05）；西药组与中西药组之间差异无显著性（P〉0．05）。结论解毒祛瘀滋阴药能增加MRL／Ipr小鼠的PBLC凋亡率并下调其线粒体跨膜电位水平。

Effects of Jiedu Quyu Ziyin Recipe on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr mice

Objective To explore the effects of Jiedu Quyu Ziyin Recipe (JQZR) on the apoptosis and expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte in MRL/lpr mice. Methods 80 MRL/lpr mice were randomly divided into model group,TCM group, Western medicine group and TCM and Western medicine group,20 mice in each group, meanwhile,20 Kunming mice were selected as normal group, then intragastrically administered normal sodium, JQZR apozem, prednisone suspension and JQZR apozem and prednisone suspension, 0. 5ml every time,once daily for 12 weeks respectively. At the end of the 12th week, peripheral-blood lymphocytes of every mice purified by gradient centrifugation were cultivated for 48 hours ,then the apoptosis was detected by flow cytometry. Furthermore,the expressions of bcl-2 and bax mRNA of peripheral-blood lymphocyte were detected by RT-PCR. Results At Oh or 48h,the apoptosis ratios of PBLC in normal group, TCM group,Western medicine group and TCM and Western medicine group are higher than model group and the differences are significant(P <0. 05 ,P <0. 01 ) ,while,at Oh,which are not significant within Western medicine group, normal group and TCM and Western medicine group ( P > 0. 05) ,even if which are significant between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group( P < 0. 05 ). At 48h, the differences of apoptosis ratios of PBLC are significant between Western medicine group and TCM and Western medicine group( P < 0. 05 ), and between TCM group and TCM and Western medicine group or between model group and TCM and Western medicine group which are significant, too( P < 0. 01 ). The ratios of bcl-2/bax mRNA of normal group,TCM group and TCM and Western medicine group are significantly lower than which of model group or Western medicine group ( P < 0. 05, P < 0. 05, P <0. 01 ,respectively) ,but there is no significant difference between which of model group and Western medicine group.Conclusion JQZR has adjustable effects on the apoptosis and expressions of bcl-2 and bax mRNA of peripheralblood lymphocyte in MRL/lpr Mice.